Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.
Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
1. | Ethyl-β-D-glucuronide-d5 (EtG-d5) | 1.21 | 200 | 226.2 | 85.0 | - |
2. | Ethyl-β-D-glucuronide (EtG) | 1.23 | 500 | 221.2 | 75.1 | 85.1 |
3. | Ethyl sulfate-d5 (EtS-d5) | 2.36 | 50 | 130.1 | 98.0 | - |
4. | Ethyl sulfate (EtS) | 2.38 | 500 | 125.1 | 97.1 | 80.0 |
Column | Raptor EtG/EtS (cat.# 9325A12) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809) | ||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||
Standard/Sample | Ethyl- | ||||||||||||||||||||
Ethyl- | |||||||||||||||||||||
Ethyl sulfate-d5 sodium salt (EtS-d5) (cat.# 34104) | |||||||||||||||||||||
Ethyl sulfate sodium salt (EtS) (cat.# 34103) | |||||||||||||||||||||
Diluent: | 0.01% Formic acid in water | ||||||||||||||||||||
Conc.: | 500 ng/mL (40x acetonitrile precipitation sample prep) | ||||||||||||||||||||
Inj. Vol.: | 10 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.01% Formic acid in water | ||||||||||||||||||||
B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Source: | Electrospray |
Ion Mode: | ESI- |
Mode: | MRM |
Instrument | HPLC |
Sample Preparation | A 500 ng/mL standard was prepared in urine. A 50 μL aliquot was mixed with 10 μL of internal standard (20 μg/mL EtG-d5 and 5 μg/mL EtS-d5 in water) and 150 µL of acetonitrile by vortexing at 3000 rpm for 10 seconds and centrifuged at 4300 rpm for 10 minutes at 10 °C. After centrifugation, 100 µL of the supernatant was diluted with 900 µL (40x dilution) of 0.01% formic acid in water. The sample was then vortexed at 3000 rpm for 10 seconds and injected for LC-MS/MS analysis. |
We have determined your location as:
Or select your location below:
Sorry! We are unable to determine your location. Please select your location below.