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Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
1. | Desmethylolanzapine | 0.58 | 2500 | 299.1 | 256.1 | 198.0 |
2. | Phenelzine sulfate | 0.59 | 2500 | 137.2 | 105.1 | 77.1 |
3. | Olanzapine | 0.65 | 2500 | 313.2 | 256.1 | 198.1 |
4. | Lamotrigine | 0.76 | 2500 | 256.0 | 211.1 | 145.0 |
5. | Molindone | 0.81 | 2500 | 278.1 | 100.3 | 101.1 |
6. | (+/-)-Hydroxybupropion | 0.83 | 2500 | 256.0 | 130.2 | 166.0 |
7. | 7-Hydroxyquetiapine | 0.84 | 2500 | 400.3 | 269.0 | 208.0 |
8. | Bupropion-D9 (IS) | 0.94 | 200 | 249.2 | 130.9 | - |
9. | Bupropion | 0.95 | 2500 | 240.0 | 184.1 | 130.2 |
10. | Venlafaxine | 1.04 | 2500 | 278.4 | 260.4 | 121.2 |
11. | Reduced haloperidol | 1.07 | 2500 | 378.1 | 359.9 | 109.1 |
12. | Milnacipran | 1.09 | 2500 | 247.2 | 100.1 | 129.1 |
13. | N-desmethylmirtazapine | 1.10 | 2500 | 252.1 | 195.1 | 209.2 |
14. | 9-Hydroxyrisperidone | 1.15 | 2500 | 427.3 | 207.1 | 110.2 |
15. | Mirtazapine | 1.16 | 2500 | 266.1 | 195.1 | 72.1 |
16. | N-desmethylclozapine | 1.19 | 2500 | 313.0 | 192.1 | 270.0 |
17. | Droperidol | 1.24 | 2500 | 380.1 | 122.9 | 165.1 |
18. | Clozapine | 1.25 | 2500 | 328.2 | 271.1 | 193.1 |
19. | Didesmethyl citalopram | 1.26 | 2500 | 297.1 | 109.1 | 261.9 |
20. | N-desmethylcitalopram | 1.27 | 2500 | 311.1 | 109.1 | 262.1 |
21. | Escitalopram | 1.28 | 2500 | 325.3 | 109.1 | 261.9 |
22. | Fluvoxamine | 1.35 | 2500 | 319.1 | 71.2 | 130.1 |
23. | Haloperidol | 1.36 | 2500 | 377.2 | 123.0 | 95.1 |
24. | Norfluoxetine | 1.37 | 2500 | 296.3 | 134.3 | 104.9 |
25. | Isocarboxazid | 1.38 | 2500 | 232.0 | 91.1 | 65.2 |
26. | Fluoxetine | 1.39 | 2500 | 310.1 | 148.0 | 115.1 |
27. | Desmethyldoxepin | 1.40 | 2500 | 266.1 | 107.1 | 115.0 |
28. | Doxepin | 1.45 | 2500 | 280.1 | 107.2 | 77.1 |
29. | Trazodone | 1.46 | 2500 | 372.3 | 176.1 | 148.0 |
30. | Oxcarbazepine | 1.51 | 2500 | 253.1 | 180.0 | 208.1 |
31. | Risperidone | 1.52 | 2500 | 411.2 | 191.0 | 110.1 |
32. | Quetiapine | 1.75 | 2500 | 384.2 | 253.0 | 221.2 |
33. | Asenapine | 1.76 | 2500 | 286.2 | 165.1 | 229.1 |
34. | Ziprasidone | 1.78 | 2500 | 413.2 | 194.1 | 130.0 |
35. | Protriptyline | 1.79 | 2500 | 264.1 | 191.1 | 165.2 |
36. | Desipramine | 1.83 | 2500 | 267.1 | 72.1 | 193.1 |
37. | Paroxetine | 1.85 | 2500 | 330.2 | 192.2 | 70.1 |
38. | Iloperidone | 1.85 | 2500 | 427.1 | 261.1 | 96.1 |
39. | Duloxetine | 1.86 | 2500 | 298.1 | 188.2 | 154.1 |
40. | Amoxapine | 1.88 | 2500 | 314.2 | 271.0 | 193.1 |
41. | Carbamazepine | 1.89 | 2500 | 237.0 | 193.9 | 192.0 |
42. | Maprotiline | 1.90 | 2500 | 278.1 | 250.2 | 191.1 |
43. | Imipramine | 1.91 | 2500 | 281.1 | 86.2 | 58.1 |
44. | Nortriptyline | 1.95 | 2500 | 264.1 | 91.1 | 115.2 |
45. | Loxapine | 1.95 | 2500 | 328.1 | 271.1 | 193.0 |
46. | Amitriptyline | 2.01 | 2500 | 278.1 | 91.1 | 202.1 |
47. | Trimipramine | 2.11 | 2500 | 295.2 | 100.2 | 58.2 |
48. | Pimozide | 2.13 | 2500 | 462.1 | 328.0 | 109.1 |
49. | Chlorpromazine | 2.26 | 2500 | 319.0 | 86.1 | 178.2 |
50. | Dehydro aripiprazole | 2.28 | 2500 | 446.2 | 285.0 | 98.1 |
51. | Clomipramine | 2.29 | 2500 | 315.3 | 86.0 | 58.0 |
52. | Sertraline | 2.30 | 2500 | 306.2 | 275.1 | 158.9 |
53. | Fluphenazine | 2.50 | 2500 | 438.3 | 171.1 | 143.2 |
54. | Aripiprazole | 2.51 | 2500 | 448.2 | 285.0 | 176.1 |
55. | Perphenazine | 2.61 | 2500 | 404.2 | 171.1 | 143.2 |
56. | Trifluoperazine | 2.63 | 2500 | 408.2 | 141.2 | 113.1 |
57. | Prochlorperazine | 2.78 | 2500 | 374.1 | 141.0 | 113.1 |
58. | Thiothixene | 2.91 | 2500 | 444.2 | 221.2 | 235.0 |
59. | Thioridazine | 3.18 | 2500 | 371.2 | 126.1 | 98.1 |
Column | Raptor Biphenyl (cat.# 9309A5E) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 3.0 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253) | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Conc.: | 2500 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water, 0.1% formic acid, 5 mM ammonium formate | ||||||||||||||||||||||||
B: | Methanol, 0.1% formic acid, 5 mM ammonium formate | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
Sample Preparation | Drug-free human urine (BioIVT) was fortified with 58 analytes at 2500 ng/mL. Bupropion-D9 was used as the internal standard for quantification of all 58 compounds. The urine sample (50 μL) was mixed with 15 μL of IMCSzyme, 20 μL of reaction buffer, and 10 μL of internal standard solution (1 µg/mL in methanol). Hydrolysis was performed at 45°C (water bath) for 30 minutes, and then 400 µL of acetonitrile was added, vortexed to mix, and centrifuged at 4000 rpm for 10 minutes. The supernatant was diluted 2-fold with water and injected for analysis. |