22 Acylcarnitines and 13 Amino Acids in Solvent on Raptor HILIC-Si EXP Guard Cartridge Column by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Phenylalanine	0.20	166.0	120.1
2.	Leucine	0.21	132.1	86.0
3.	Isoleucine	0.21	132.1	86.1
4.	Tyrosine	0.22	182.1	91.0
5.	Methionine	0.24	150.1	56.1
6.	Valine	0.27	118.1	72.0
7.	C20-Eicosanoyl-L-carnitine	0.27	456.4	85.1
8.	C18-Stearoyl-L-carnitine	0.28	428.3	85.1
9.	C18:1 Oleoyl-L-carnitine	0.29	426.4	85.1
10.	C18:2 Linoleoyl-L-carnitine	0.30	424.3	85.1
11.	C16-Palmitoyl-L-carnitine	0.30	400.3	85.1
12.	C16:1 Palmitolelyl-L-carnitine	0.31	398.3	85.1
13.	C14-Myristoyl-L-carnitine	0.32	372.3	85.1
14.	C14:1 Tetradecenoyl-L-carnitine	0.33	370.3	85.1
15.	C14:2-Tetradecadienoyl-L-carnitine	0.33	368.3	85.1
16.	Proline	0.33	116.0	70.1
17.	C12-Lauroyl-L-carnitine	0.35	344.3	85.1

	Peaks	t_R (min)	Precursor Ion	Product Ion
18.	Alanine	0.36	90.1	44.1
19.	C10-Decanoyl-L-carnitine	0.38	316.3	85.1
20.	C8-Octanoyl-L-carnitine	0.43	288.3	85.1
21.	C7-Heptanoyl-L-carnitine	0.45	274.2	85.1
22.	C6-Hexanoyl-L-carnitine	0.47	260.2	85.1
23.	Glutamine	0.45	147.1	84.1
24.	C5-Valeryl-L-carnitine	0.48	246.2	85.1
25.	C5-Isovaleryl-L-carnitine	0.49	246.1	85.1
26.	2-Methylbutyryl-L-carnitine	0.49	246.2	85.1
27.	C5:1-Tiglyl-L-carnitine	0.50	244.2	85.1
28.	C4-Butyryl-L-carnitine	0.51	232.2	85.1
29.	C4-Isobutyryl-L-carnitine	0.51	232.1	85.1
30.	Citrulline	0.51	176.1	113.1
31.	Glutamic acid	0.55	148.1	83.9
32.	C3-Propionyl-L-carnitine	0.54	218.1	85.1
33.	C2-Acetyl-L-carnitine	0.56	204.1	85.1
34.	Arginine	0.66	175.2	70.1
35.	Ornithine	0.69	133.1	70.1

Internal standard peaks not shown.

Column

Raptor HILIC-Si EXP guard cartridge column (cat.# 9310A0252)

Dimensions:

5 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

45 °C

Standard/Sample

Diluent:

85:15 Acetonitrile:water (v/v)

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

30 mM Ammonium formate in water

Acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	15	85
0.4	0.5	70	30
0.41	0.5	15	85
1.20	0.5	15	85

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Instrument	4500
Sample Preparation	A 100 ng/mL standard mix of 22 acylcarnitines and 13 amino acids was prepared in 85:15 acetonitrile:water (v/v) that was fortified with known concentrations of stable isotope-labeled internal standards. The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis.

Download PDF