Analysis of Remdesivir and Metabolites on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion	Product Ion
1.	Remdesivir nucleoside monophosphate	0.8	500	372.1	202.1	147.2	229.1
2.	GS-704277	1.1	500	443.1	202.2	124.1	229.2
3.	GS-441524	2.1	500	292.0	202.1	163.1	147.1
4.	Remdesivir	2.9	50	603.2	200.1	229.1	402.2

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

0.1% Acetic acid in water

Inj. Vol.:

2 µL

Mobile Phase

Water, 0.1% acetic acid, 20 mM ammonium acetate

Methanol, 0.1% acetic acid, 20 mM ammonium acetate

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	100	0
1.50	0.5	45	55
2.00	0.5	0	100
3.00	0.5	0	100
3.01	0.5	100	0
4.50	0.5	100	0

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	Aliquot 50 μL of plasma and add 200 μL of cold acetonitrile containing analytes. Vortex for 30 seconds and centrifuge for 10 minutes at 4200 rpm. Transfer 100 μL to vial and dry off solvent. Reconstitute with 100 μL of 0.1% acetic acid in water and vortex.

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