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|Column||Raptor HILIC-Si (cat.# 9310A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Diluent:||10 mM Ammonium formate in 100% methanol|
|Inj. Vol.:||2 µL|
|A:||Water, 10 mM ammonium formate|
|B:||90:10 Acetonitrile:water (v/v), 10 mM ammonium formate|
|Sample Preparation||A 100 ng/mL standard mix of psilocin and psilocybin was prepared in 10 mM ammonium formate in 100% methanol. The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis.|