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|Column||Raptor Biphenyl (cat.# 9309A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor Biphenyl EXP guard column cartridge 5.0 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)|
|Diluent:||Water, 0.1% formic acid + 2 mM ammonium formate|
|Inj. Vol.:||5 µL|
|A:||Water, 0.1% formic acid + 2 mM ammonium formate|
|B:||Methanol, 0.1% formic acid + 2 mM ammonium formate|
|Sample Preparation||A 500 ng/mL standard mix of psilocin and psilocybin was prepared in pooled urine. A 50 µL aliquot was taken from the standard and mixed with 10 µL of internal standard (psilocin-D10, 20 µg/mL) and 100 µL of methanol. The mixture was vortexed at 3000 rpm for 10 seconds and centrifuged at 4300 rpm for 10 minutes at 10 °C. After centrifugation, 100 µL of the supernatant was diluted with 900 µL (20-fold dilution) of water containing 0.1% formic acid and 2 mM ammonium formate (mobile phase A) and injected for LC-MS/MS analysis.|