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|Peaks||tR (min)||Precursor||Product 1||Product 2|
|2.||Ethyl sulfate (EtS)||0.64||124.9||96.9||79.9|
|Column||Force Biphenyl (cat.# 962935E)|
|Dimensions:||50 mm x 3.0 mm ID|
|Particle Size:||3 µm|
|Pore Size:||100 Å|
|Guard Column:||Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253)|
|Standard/Sample||Ethyl-β-D-glucuronide (EtG) (cat.# 34101)|
|Ethyl sulfate sodium salt (EtS) (cat.# 34103)|
|Conc.:||50 ng/mL in urine|
|Inj. Vol.:||10 µL|
|A:||Water, 0.1% formic acid|
|B:||Methanol, 0.1% formic acid|
|Sample Preparation||A 100 μL aliquot of urine was spiked at 50 ng/mL with EtG and EtS and then diluted with 900 μL of MS water in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.# 24498). The sample was vortexed for 10 seconds and injected for LC-MS/MS analysis.|
|Notes||The analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809).|
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