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Alcohol Metabolites on Force Biphenyl by LC-MS/MS

PeakstR (min)PrecursorProduct 1Product 2
1.Urinary interference0.41---
2.Ethyl sulfate (EtS)0.64124.996.979.9
3.Ethyl-β-D-glucuronide (EtG)1.03221.075.185.0
ColumnForce Biphenyl (cat.# 962935E)
Dimensions:50 mm x 3.0 mm ID
Particle Size:3 µm
Pore Size:100 Å
Guard Column:Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253)
Temp.:30 °C
Standard/SampleEthyl-β-D-glucuronide (EtG) (cat.# 34101)
Ethyl sulfate sodium salt (EtS) (cat.# 34103)
Diluent:1:9 Urine:water
Conc.:50 ng/mL in urine
Inj. Vol.:10 µL
Mobile Phase
A:Water, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
Ion Source:Electrospray
Ion Mode:ESI-
Sample PreparationA 100 μL aliquot of urine was spiked at 50 ng/mL with EtG and EtS and then diluted with 900 μL of MS water in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.# 24498). The sample was vortexed for 10 seconds and injected for LC-MS/MS analysis.
NotesThe analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809).