Alcohol Metabolites on Force Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor	Product 1	Product 2
1.	Urinary interference	0.41	-	-	-
2.	Ethyl sulfate (EtS)	0.64	124.9	96.9	79.9
3.	Ethyl-β-D-glucuronide (EtG)	1.03	221.0	75.1	85.0

Column

Force Biphenyl (cat.# 962935E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Guard Column:

Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253)

Temp.:

30 °C

Standard/Sample

Ethyl-β-D-glucuronide (EtG) (cat.# 34101)

Ethyl sulfate sodium salt (EtS) (cat.# 34103)

Diluent:

1:9 Urine:water

Conc.:

50 ng/mL in urine

Inj. Vol.:

10 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	100	0
3.00	0.8	5	95
3.01	0.8	0	100
3.50	0.8	0	100
3.51	0.8	100	0
5.00	0.8	100	0

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Instrument	UHPLC
Sample Preparation	A 100 μL aliquot of urine was spiked at 50 ng/mL with EtG and EtS and then diluted with 900 μL of MS water in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.# 24498). The sample was vortexed for 10 seconds and injected for LC-MS/MS analysis.
Notes	The analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809).

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