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Peaks | tR (min) | Precursor | Product 1 | Product 2 | |
---|---|---|---|---|---|
1. | Urinary interference | 0.41 | - | - | - |
2. | Ethyl sulfate (EtS) | 0.64 | 124.9 | 96.9 | 79.9 |
3. | Ethyl-β-D-glucuronide (EtG) | 1.03 | 221.0 | 75.1 | 85.0 |
Column | Force Biphenyl (cat.# 962935E) | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 3.0 mm ID | ||||||||||||||||||||||||||||
Particle Size: | 3 µm | ||||||||||||||||||||||||||||
Pore Size: | 100 Å | ||||||||||||||||||||||||||||
Guard Column: | Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253) | ||||||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||||||
Standard/Sample | Ethyl-β-D-glucuronide (EtG) (cat.# 34101) | ||||||||||||||||||||||||||||
Ethyl sulfate sodium salt (EtS) (cat.# 34103) | |||||||||||||||||||||||||||||
Diluent: | 1:9 Urine:water | ||||||||||||||||||||||||||||
Conc.: | 50 ng/mL in urine | ||||||||||||||||||||||||||||
Inj. Vol.: | 10 µL | ||||||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||||||
A: | Water, 0.1% formic acid | ||||||||||||||||||||||||||||
B: | Methanol, 0.1% formic acid | ||||||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Source: | Electrospray |
Ion Mode: | ESI- |
Instrument | UHPLC |
Sample Preparation | A 100 μL aliquot of urine was spiked at 50 ng/mL with EtG and EtS and then diluted with 900 μL of MS water in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.# 24498). The sample was vortexed for 10 seconds and injected for LC-MS/MS analysis. |
Notes | The analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809). |
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