Drug and Drug Metabolites on Force Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Norcotinine	1.35	163.1	80.1	118.0
2.	Nicotine	1.39	163.2	130.1	117.1
3.	BZP	1.44	177.2	91.1	65.1
4.	Anabasine	1.51	163.2	92.1	120.1
5.	Normorphine	1.56	272.3	165.1	152.0
6.	Noroxymorphone	1.80	288.2	270.2	213.2
7.	Lisdexamfetamine	1.82	264.2	84.2	91.2
8.	Cotinine	2.02	177.2	80.1	98.1
9.	Psilocybin	2.04	285.3	205.2	58.3
10.	Dihydromorphine	2.11	288.3	157.1	185.0
11.	Morphine	2.16	286.2	152.1	165.1
12.	Morphine-6-β-D-glucuronide	2.22	462.3	286.2	201.1
13.	Oxymorphone	2.23	302.2	227.1	198.1
14.	Morphine-N-oxide	2.31	302.3	285.2	162.1
15.	Hydromorphone	2.41	286.2	185.1	157.2
16.	Psilocin	2.42	205.2	58.3	160.1
17.	Olanzapine	2.66	313.2	256.1	198.1
18.	Methylephedrine	2.68	180.2	162.2	115.2
19.	Norcodeine	2.69	286.1	152.1	115.0
20.	Naloxone	2.90	328.3	271.1	310.1
21.	Methamphetamine	2.93	150.3	91.1	119.2
22.	Noroxycodone	2.98	302.1	284.2	227.1
23.	Dihydrocodeine	2.98	302.2	199.2	128.0
24.	MDA	2.98	180.2	163.1	105.2
25.	Codeine	3.02	300.2	152.0	165.0
26.	O-Desmethyltramadol	3.02	250.1	44.0	232.0
27.	6-Acetylmorphine	3.04	328.2	165.1	211.2
28.	Norhydrocodone	3.05	286.2	199.1	128.2
29.	Phentermine	3.07	150.3	91.1	133.2
30.	Methylone	3.07	208.1	160.2	132.2
31.	Desomorphine	3.18	272.1	165.0	152.2
32.	Oxycodone	3.19	316.2	298.2	169.0
33.	Naltrexone	3.24	342.2	324.3	267.2
34.	Hydrocodone	3.27	300.2	199.2	128.2
35.	Levamisole	3.31	205.1	178.1	91.2
36.	7-Aminonitrazepam	3.34	252.2	121.1	94.2
37.	O-Desmethylvenlafaxine	3.38	264.2	58.2	107.0
38.	MDEA	3.54	208.2	163.2	105.2
39.	m-Hydroxybenzoylecgonine	3.59	306.2	168.2	121.1
40.	Lamotrigine	3.62	256.1	210.9	145.0
41.	N-Desmethyltapentadol	3.63	208.1	107.2	121.2
42.	Tapentadol	3.76	222.2	107.2	121.2
43.	Hydroxybupropion	3.80	256.0	166.0	130.2
44.	Molindone	3.81	278.1	100.1	70.1
45.	Zolpidem Phenyl-4-carboxylic acid	3.82	338.2	265.1	219.0
46.	Dextrorphan	3.84	258.2	157.1	201.2
47.	Levorphanol	3.84	258.2	157.2	199.2
48.	Tramadol	3.86	264.2	58.2	77.0
49.	N-Desmethyltramadol	3.98	250.2	44.1	232.1
50.	Methylphenidate	4.00	234.4	84.2	56.1
51.	Lacosamide	4.03	251.5	91.2	108.2
52.	Normeperidine	4.06	234.1	160.2	91.0
53.	Benzoylecgonine	4.11	290.2	168.2	77.2
54.	Remifentanil	4.13	377.4	113.2	317.2
55.	Cocaine	4.20	304.2	182.2	77.1
56.	7-Aminoclonazepam	4.24	286.1	121.2	250.1
57.	N-Desmethylclozapine	4.25	313.0	192.1	270.1
58.	N-Desmethylmirtazapine	4.27	252.1	195.2	209.2
59.	Norcocaine	4.29	290.1	168.2	136.2
60.	MDPV	4.29	276.2	126.3	135.2
61.	Pentazocine	4.38	286.2	218.2	69.1
62.	Mirtazapine	4.40	266.1	195.2	107.2
63.	Butorphanol	4.47	328.3	310.2	131.0
64.	Clozapine	4.49	328.2	271.0	193.0

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
65.	Paliperidone	4.51	427.4	207.2	110.1
66.	Iloperidone	4.51	427.1	207.2	110.2
67.	9-Hydroxyrisperidone	4.51	427.0	207.2	110.2
68.	Didemethylcitalopram	4.73	297.1	109.2	262.1
69.	Citalopram	4.75	325.2	109.2	262.1
70.	Escitalopram	4.75	325.3	109.2	262.1
71.	Topiramate	4.76	340.0	264.1	224.0
72.	Diphenhydramine	4.76	256.2	167.2	152.1
73.	Haloperidol	4.80	377.2	123.1	95.0
74.	Desmethyldoxepin	4.82	266.1	115.0	107.2
75.	Ezogabine	4.84	304.1	109.2	230.1
76.	Fluoxetine	4.91	310.1	44.1	148.0
77.	Dextromethorphan	5.01	272.2	215.2	171.1
78.	Chlordiazepoxide	5.05	300.1	227.1	282.0
79.	Norpropoxyphene	5.08	326.4	252.2	91.0
80.	Propoxyphene	5.09	340.3	58.2	266.3
81.	Asenapine	5.11	286.2	166.0	229.0
82.	Midazolam	5.16	326.1	291.2	223.0
83.	Sufentanil	5.16	387.3	238.2	111.1
84.	Protriptyline	5.18	264.1	191.1	165.0
85.	Promazine	5.19	285.1	86.2	58.2
86.	Amoxapine	5.21	314.2	271.1	193.1
87.	Oxcarbazepine	5.22	253.1	180.1	208.1
88.	Paroxetine	5.23	330.2	192.2	70.2
89.	Phenytoin	5.24	253.0	182.2	104.2
90.	Loxapine	5.25	328.2	271.1	193.1
91.	Maprotiline	5.26	278.1	250.3	191.1
92.	Cyclobenzaprine	5.26	276.2	215.1	189.0
93.	Amitriptyline	5.27	278.2	202.1	91.2
94.	EDDP	5.27	278.2	234.1	249.2
95.	Venlafaxine	5.27	278.4	260.2	121.2
96.	Nortriptyline	5.29	264.1	91.0	115.0
97.	Tenoxicam	5.32	338.1	121.2	95.0
98.	Trimipramine	5.40	295.2	100.2	58.3
99.	Pimozide	5.43	462.1	109.2	328.2
100.	Mesoridazine	5.44	387.3	126.1	98.2
101.	α-Hydroxymidazolam	5.48	342.2	324.1	203.1
102.	Clomipramine	5.53	315.3	86.2	58.2
103.	Methadone	5.55	310.2	265.2	105.2
104.	Sertraline	5.56	306.1	158.9	275.1
105.	Metaxalone	5.61	222.1	161.2	77.1
106.	Eslicarbazepine	5.65	297.2	194.2	237.1
107.	EMDP	5.68	264.2	220.1	235.2
108.	N-Desmethylflunitrazepam	5.82	300.1	254.1	198.1
109.	Ketorolac	5.89	256.1	105.2	77.1
110.	Etoricoxib	5.95	359.2	280.1	279.1
111.	Clonazepam	5.97	316.1	270.1	214.1
112.	Tolmetin	6.03	258.1	119.2	91.1
113.	Thioridazine	6.03	371.3	126.3	98.2
114.	α-Hydroxytriazolam	6.04	359.2	331.1	176.0
115.	Methaqualone	6.10	251.1	132.1	91.2
116.	α-Hydroxyalprazolam	6.17	325.2	297.1	216.2
117.	Clobazam	6.28	301.0	259.1	224.1
118.	Parecoxib	6.32	371.2	234.1	165.0
119.	Zaleplon	6.32	306.2	236.1	264.2
120.	Flunitrazepam	6.39	314.2	268.1	239.1
121.	Temazepam	6.43	301.1	255.2	283.1
122.	Estazolam	6.44	295.1	267.1	205.1
123.	Diazepam	6.74	285.1	193.1	154.2
124.	XLR-11	7.02	330.3	125.1	144.1
125.	Prazepam	7.04	325.2	271.2	140.1
126.	THC	7.15	315.2	193.0	123.1
127.	JWH-073	7.54	328.2	127.1	155.1
128.	JWH-018	7.64	342.3	155.2	127.1

Column

Force Biphenyl (cat.# 962935E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Guard Column:

Force Biphenyl EXP guard column cartridge 5 mm, 3 mm ID, 5 µm (cat.# 962950253)

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Conc.:

80 ng/mL

Inj. Vol.:

1 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	96	4
7.00	0.8	0	100
8.00	0.8	0	100
8.01	0.8	96	4
10.00	0.8	96	4

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Instrument	UHPLC
Sample Preparation	Standards were prepared in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.#24498).
Notes	The analytical and guard columns were equipped with an UltraShield precolumn filter (cat. 25809).

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