Separation of 25 Acylcarnitines on Raptor ARC-18

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	C0-L-Carnitine	0.40	162.2	85.1
2.	C2-Acetyl-L-carnitine	0.59	204.2	85.1
3.	Methyl-malonyl-L-carnitine	1.15	262.3	85.1
4.	C3-Propionyl-L-carnitine	1.29	218.1	85.1
5.	3-Hydroxyisovaleryl-L-carnitine	1.60	262.4	85.1
6.	C4-Isobutyrl-L-carnitine	2.31	232.1	85.1
7.	C4-Butyryl-L-carnitine	2.42	232.2	85.1
8.	3-Methylcrotonyl-L-carnitine	3.31	244.1	85.1
9.	C5:1-Tigyl-L-carnitine	3.49	244.2	85.1
10.	2-Methylbutyryl-L-carnitine	3.61	246.1	85.1
11.	C5-Isovaleryl-L-carnitine	3.71	246.1	85.1
12.	C5-Valeryl-L-carnitine	3.87	246.2	85.1

	Peaks	t_R (min)	Precursor Ion	Product Ion
13.	C6-Hexanoyl-L-carnitine	5.08	260.3	85.1
14.	C7-Heptanoyl-L-carnitine	5.27	274.2	85.1
15.	C8-Octanoyl-L-carnitine	5.41	288.4	85.1
16.	C10-Decanoyl-L-carnitine	5.67	316.3	85.1
17.	C14:2-Tetradecadienoyl-L-carnitine	5.90	368.5	85.1
18.	C12-Lauroyl-L-carnitine	5.94	344.5	85.1
19.	C14:1-Tetradecanoyl-L-carnitine	6.17	369.8	85.1
20.	C14-Myristoyl-L-carnitine	6.25	372.4	85.1
21.	C18:2 Linoleoyl-L-carnitine	6.42	424.1	85.1
22.	C16:1 Palmitolelyl-L-carnitine	6.58	398.4	85.1
23.	C16-Palmitoyl-L-carnitine	6.69	400.5	85.1
24.	C18:1 Oleoyl-L-carnitine	6.77	426.4	85.1
25.	C18 - Stearoyl-L-carnitine	7.13	429.1	85.1

Column

Raptor ARC-18 (cat.# 9314A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP Guard Column Cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

35 °C

Standard/Sample

Diluent:

Water, 0.1% formic acid

Conc.:

100 ng/mL

Inj. Vol.:

3 µL

Mobile Phase

Water, 0.1% formic acid

90:10 Acetonitrile:isopropanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	98	2
1.00	0.6	98	2
4.00	0.6	88	12
6.00	0.6	0	100
7.50	0.6	0	100
8.00	0.6	98	2
9.00	0.6	98	2

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	A 100 ng/mL standard mix of all of the acylcarnitines was prepared in plasma. A 100 µL aliquot was taken from the standard and mixed with 300 µL of methanol, vortexed for 10 seconds, and then centrifuged for 10 minutes at 4000 rpm. 100 µL of the supernatant was added to a 2 mL vial (cat.# 24619) containing 900 µL of MPA (0.1% formic acid in water), capped with a short screw cap (cat.# 24498), and injected for LC-MS/MS analysis.

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