Aldehyde-Ketone-DNPH TO-11A Calibration Mix on Raptor C18

Column

Raptor C18 (cat.# 9304A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

30 °C

Standard/Sample

Aldehyde-ketone-DNPH TO-11A calibration mix (cat.# 31808)

Diluent:

Acetonitrile

Conc.:

15 µg/mL

Inj. Vol.:

2 µL

Mobile Phase

Water

Methanol:acetonitrile (650:50)*

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	30	70
5.00	0.8	25	75
11.00	0.8	10	90
11.01	0.8	0	100
12.00	0.8	0	100
12.01	0.8	30	70
14.00	0.8	30	70

Detector	UV/Vis @ 365, 4.8 nm
Notes	*Mobile phase B was prepared by combining 650 mL methanol and 50 mL acetonitrile.

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