Perfluorooctanoic Acid (PFOA) and Perfluorooctanesulfonic Acid (PFOS) on Raptor C18 by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluoro-[1,2-¹³C₂]octanoic acid (¹³C-PFOA)	1.38	10	414.8	369.8
2.	Perfluorooctanoic acid (PFOA)	1.38	5	412.7	368.8
3.	Perfluoro-1-[1,2,3,4-¹³C₄]octanesulfonic acid (¹³C-PFOS)	1.65	10	502.7	79.9
4.	Perfluorooctanesulfonic acid (PFOS)	1.65	5	498.7	79.9

Column

Raptor C18 (cat.# 9304512)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Temp.:

40 °C

Sample

Diluent:

Water:methanol (50:50)

Conc.:

5-10 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

5 mM ammonium acetate in water

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	40	60
2.50	0.4	5	95
2.51	0.4	40	60
4.50	0.4	40	60

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