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Perfluorooctanoic Acid (PFOA) and Perfluorooctanesulfonic Acid (PFOS) on Raptor C18 by LC-MS/MS

LC_EV0536
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
1.Perfluoro-[1,2-13C2]octanoic acid (13C-PFOA)1.3810414.8369.8
2.Perfluorooctanoic acid (PFOA)1.385412.7368.8
3.Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C-PFOS)1.6510502.779.9
4.Perfluorooctanesulfonic acid (PFOS)1.655498.779.9
ColumnRaptor C18 (cat.# 9304512)
Dimensions:100 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Temp.:40 °C
Sample
Diluent:Water:methanol (50:50)
Conc.:5-10 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:5 mM ammonium acetate in water
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.44060
2.500.4595
2.510.44060
4.500.44060
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC