Perfluorinated Alkyl Acids on Raptor C18 by LC-MS/MS for EPA Method 537

LC_EV0548

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	1.98	10	299.10	79.99
2.	Perfluoro-n-[1,2-¹³C₂]hexanoic acid (¹³C-PFHxA)	2.92	10	315.13	270.13
3.	Perfluorohexanoic acid (PFHxA)	2.93	10	313.10	269.12
4.	Perfluoroheptanoic acid (PFHpA)	4.00	5	363.16	319.09
5.	Perfluorohexanesulfonic acid (PFHxS)	4.14	10	399.13	79.98
6.	Perfluoro-[1,2-¹³C₂]octanoic acid (¹³C-PFOA)	4.83	5	415.13	370.10
7.	Perfluorooctanoic acid (PFOA)	4.83	5	413.16	369.10
8.	Perfluorononanoic acid (PFNA)	5.50	5	463.16	419.19
9.	Perfluoro-1-[1,2,3,4-¹³C₄]octanesulfonic acid (¹³C-PFOS)	5.54	5	503.13	79.98
10.	Perfluorooctanesulfonic acid (PFOS)	5.54	10	499.17	79.98

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
11.	Perfluoro-n-[1,2-¹³C₂]decanoic acid (¹³C-PFDA)	6.06	5	515.17	470.17
12.	Perfluorodecanoic acid (PFDA)	6.07	5	513.17	469.16
13.	N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)	6.28	5	573.23	419.15
14.	N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)	6.28	5	570.20	419.17
15.	N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)	6.52	5	589.27	419.11
16.	N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)	6.52	5	584.20	419.18
17.	Perfluoroundecanoic acid (PFUnA)	6.56	5	563.23	519.24
18.	Perfluorododecanoic acid (PFDoA)	6.98	5	613.23	569.19
19.	Perfluorotridecanoic acid (PFTrDA)	7.35	5	663.23	619.21
20.	Perfluorotetradecanoic acid (PFTA)	7.67	5	713.23	669.23

Column

Raptor C18 (cat.# 9304552)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

5 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

96:4 Methanol:water

Conc.:

5–10 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

5 mM ammonium acetate in water

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	75	25
8.00	0.4	15	85
8.01	0.4	75	25
9.00	0.4	75	25

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Waters ACQUITY

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