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Perfluorinated Compounds (PFCs) on Raptor C18 2.7 μm

LC_EV0551
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
1.Perfluorobutanoic acid (PFBA)0.885213.1169.1
2.Perfluoropentanoic acid (PFPeA)1.815263.1219.1
3.Perfluorobutane sulfonic acid (PFBS)2.075299.180.0
4.1H, 1H, 2H, 2H-Perfluorohexane sulfonic acid (4:2 FTS)2.615327.1307.1
5.Perfluorohexanoic acid (PFHxA)2.695313.1269.1
6.Perfluoropentane sulfonic acid (PFPeS)2.825349.180.0
7.Perfluoroheptanoic acid (PFHpA)3.315363.2319.1
8.Perfluorohexane sulfonic acid (PFHxS)3.375399.180.0
9.2-Perfluorohexyl ethanoic acid (FHEA)3.43250377.1293.1
10.1H, 1H, 2H, 2H-Perfluorooctane sulfonic acid (6:2 FTS)3.745427.2407.2
11.Perfluorooctanoic acid (PFOA)3.775413.2369.1
12.Perfluoroheptane sulfonic acid (PFHpS)3.805449.280.0
13.Perfluorononanoic acid (PFNA)4.155463.2419.2
14.Perfluorooctane sulfonic acid (PFOS)4.165499.280.0
15.2-Perfluorooctyl ethanoic acid (FOEA)4.26250477.2393.2
16.1H, 1H, 2H, 2H-Perfluorodecane sulfonic acid (8:2 FTS)4.455527.2507.2
17.Perfluorodecanoic acid (PFDA)4.475513.2469.2
PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct Ion
18.Perfluorononane sulfonic acid (PFNS)4.485549.280.0
19.N-Methyl perfluorooctane sulfonamidoacetic acid (N-MeFOSAA)4.605570.2419.2
20.Perfluorodecane sulfonic acid (PFDS)4.745599.280.0
21.N-Ethyl perfluorooctane sulfonamidoacetic acid (N-EtFOSAA)4.745584.2419.2
22.Perfluoroundecanoic acid (PFUnA)4.745563.2519.2
23.Perfluorooctane sulfonamide (FOSA)4.805498.278.0
24.2-Perfluorodecyl ethanoic acid (FDEA)4.84250577.2493.2
25.Perfluorododecanoic acid (PFDoA)4.985613.2569.2
26.1H, 1H, 2H, 2H-Perfluorododecane sulfonic acid (10:2 FTS)4.995627.2607.2
27.Perfluorotridecanoic acid (PFTrDA)5.195663.2619.2
28.N-Methyl perfluorooctane sulfonamide (N-MeFOSA)5.325512.2169.1
29.N-Methyl perfluorooctane sulfonamidoethanol (N-MeFOSE)5.3250616.359.1
30.Perfluorotetradecanoic acid (PFTeDA)5.375713.2669.2
31.N-Ethyl perfluorooctane sulfonamide (N-EtFOSA)5.485526.2169.1
32.N-Ethyl perfluorooctane sulfonamidoethanol (N-EtFOSE)5.4850630.359.1
33.Perfluorohexadecanoic acid (PFHxDA)5.675813.3769.3
34.Perfluorooctadecanoic acid (PFODA)5.895913.3869.3
ColumnRaptor C18 (cat.# 9304A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Standard/Sample
Diluent:80:20 Water:methanol
Conc.:5-250 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.48020
6.000.4595
6.500.4595
6.510.48020
8.000.48020
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC