Perfluorinated Compounds (PFCs) on Force C18 (1.8 μm)

LC_EV0552

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluorobutanoic acid (PFBA)	1.33	5	213.1	169.1
2.	Perfluoropentanoic acid (PFPeA)	2.38	5	263.1	219.1
3.	Perfluorobutane sulfonic acid (PFBS)	2.58	5	299.1	80.0
4.	1H, 1H, 2H, 2H-Perfluorohexane sulfonic acid (4:2 FTS)	3.11	5	327.1	307.1
5.	Perfluorohexanoic acid (PFHxA)	3.17	5	313.1	269.1
6.	Perfluoropentane sulfonic acid (PFPeS)	3.27	5	349.1	80.0
7.	Perfluoroheptanoic acid (PFHpA)	3.74	5	363.2	319.1
8.	Perfluorohexane sulfonic acid (PFHxS)	3.79	5	399.1	80.0
9.	2-Perfluorohexyl ethanoic acid (FHEA)	3.88	250	377.1	293.1
10.	1H, 1H, 2H, 2H-Perfluorooctane sulfonic acid (6:2 FTS)	4.16	5	427.2	407.2
11.	Perfluorooctanoic acid (PFOA)	4.18	5	413.2	369.1
12.	Perfluoroheptane sulfonic acid (PFHpS)	4.20	5	449.2	80.0
13.	Perfluorononanoic acid (PFNA)	4.54	5	463.2	419.2
14.	Perfluorooctane sulfonic acid (PFOS)	4.54	5	499.2	80.0
15.	2-Perfluorooctyl ethanoic acid (FOEA)	4.66	250	477.2	393.2
16.	1H, 1H, 2H, 2H-Perfluorodecane sulfonic acid (8:2 FTS)	4.84	5	527.2	507.2
17.	Perfluorodecanoic acid (PFDA)	4.84	5	513.2	469.2

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
18.	Perfluorononane sulfonic acid (PFNS)	4.84	5	549.2	80.0
19.	N-Methyl perfluorooctane sulfonamidoacetic acid (N-MeFOSAA)	4.97	5	570.2	419.2
20.	Perfluorodecane sulfonic acid (PFDS)	5.09	5	599.2	80.0
21.	N-Ethyl perfluorooctane sulfonamidoacetic acid (N-EtFOSAA)	5.10	5	584.2	419.2
22.	Perfluoroundecanoic acid (PFUnA)	5.10	5	563.2	519.2
23.	Perfluorooctane sulfonamide (FOSA)	5.19	5	498.2	78.0
24.	2-Perfluorodecyl ethanoic acid (FDEA)	5.20	250	577.2	493.2
25.	Perfluorododecanoic acid (PFDoA)	5.32	5	613.2	569.2
26.	1H, 1H, 2H, 2H-Perfluorododecane sulfonic acid (10:2 FTS)	5.33	5	627.2	607.2
27.	Perfluorotridecanoic acid (PFTrDA)	5.51	5	663.2	619.2
28.	N-Methyl perfluorooctane sulfonamide (N-MeFOSA)	5.68	5	512.2	169.1
29.	N-Methyl perfluorooctane sulfonamidoethanol (N-MeFOSE)	5.68	50	616.3	59.1
30.	Perfluorotetradecanoic acid (PFTeDA)	5.68	5	713.2	669.2
31.	N-Ethyl perfluorooctane sulfonamide (N-EtFOSA)	5.83	5	526.2	169.1
32.	N-Ethyl perfluorooctane sulfonamidoethanol (N-EtFOSE)	5.83	50	630.3	59.1
33.	Perfluorohexadecanoic acid (PFHxDA)	5.94	5	813.3	769.3
34.	Perfluorooctadecanoic acid (PFODA)	6.15	5	913.3	869.3

Column

Force C18 (cat.# 9634252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Pore Size:

100 Å

Temp.:

40 °C

Standard/Sample

Diluent:

80:20 Water:methanol

Conc.:

5-250 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

Water, 5 mM ammonium acetate

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	80	20
6.00	0.4	5	95
6.50	0.4	5	95
6.51	0.4	80	20
8.00	0.4	80	20

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	UHPLC

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