Ultra-short Chain, Legacy, and Alternative PFAS on Raptor C18 in Reagent Water

LC_EV0555

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion
1.	Perfluoropropanoic acid (PFPrA)	2.74	80	162.9	119.0
2.	Perfluorobutanoic acid (PFBA)	4.69	80	212.8	169.0
3.	Perfluoropropanesulfonic acid (PFPrS)	5.13	80	248.8	79.6
4.	Perfluorobutanesulfonic acid (PFBS)	6.14	80	298.8	79.9
5.	Perfluoro-n-[1,2-¹³C₂]hexanoic acid (¹³C₂-PFHxA)	6.75	50	314.9	270.0
6.	Hexafluoropropylene oxide-dimer acid (HFPO-DA)	6.92	80	285.0	168.9

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion
7.	Ammonium 4,8-dioxa-3H-perfluorononanoate (ADONA)	7.33	80	376.9	250.7
8.	Perfluorooctanoic acid (PFOA)	7.70	80	413.1	368.9
9.	Perfluoro-[1,2-¹³C₂]octanoic acid (¹³C₂-PFOA)	7.70	50	415.0	370.0
10.	Perfluorooctanesulfonic acid (PFOS)	8.01	80	498.8	80.0
11.	Perfluoro-[1,2,3,4-¹³C₄]octanesulfonic acid (¹³C₄-PFOS)	8.01	50	503.0	80.0
12.	9-Chlorohexadecafluoro-3-oxanonane-1-sulfonate (9Cl-PF3ONS)	8.15	80	530.8	350.7
13.	11-Chloroeicosafluoro-3-oxanonane-1-sulfonate (11Cl-PF3OUdS)	8.61	80	630.7	451.0

Column

Raptor C18 (cat.# 9304A1E)

Dimensions:

100 mm x 3 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Conc.:

80 ppt

Inj. Vol.:

10 µL

Mobile Phase

A:

Water, 5 mM ammonium acetate

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.25	80	20
7.00	0.25	5	95
9.00	0.25	5	95
9.01	0.25	80	20
11.0	0.25	80	20

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	UHPLC
Sample Preparation	In a polypropylene vial, 250 µL of reagent water (fortified at 80 ppt) was mixed with 250 µL of 40:60 reagent water:methanol and 5 µL of internal standard solution (5 ng/mL of ¹³C₂-PFHxA, ¹³C₂-PFOA, ¹³C₄-PFOS in methanol). The vial was capped with a polyethylene cap prior to analysis.
Notes	A PFAS delay column (cat.# 27854) was installed between the pump mixer and the injector.

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