EPA Method 537.1 on Raptor C18

LC_EV0560

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	2.06	10	298.8	79.9
2.	Perfluoro-n-[1,2-¹³C₂]hexanoic acid (¹³C₂-PFHxA)	3.03	5	314.9	270.0
3.	Perfluorohexanoic acid (PFHxA)	3.04	5	312.9	268.7
4.	Tetrafluoro-2-heptafluoropropoxy-¹³C₃-propanoic acid (¹³C₃-HFPO-DA)	3.36	5	286.8	168.7
5.	Hexafluoropropylene oxide dimer acid (HFPO-DA)	3.38	5	285.0	168.9
6.	Perfluoroheptanoic acid (PFHpA)	4.09	5	362.8	318.8
7.	Perfluorohexanesulfonic acid (PFHxS)	4.22	10	398.8	79.9
8.	4,8-Dioxa-3H-perfluorononanoic acid (ADONA)	4.24	5	376.9	250.7
9.	Perfluoro-[1,2-¹³C₂]octanoic acid (¹³C₂-PFOA)	4.88	5	415.0	370.0
10.	Perfluorooctanoic acid (PFOA)	4.90	5	413.1	368.9
11.	Perfluorononanoic acid (PFNA)	5.54	5	462.9	418.9
12.	Perfluoro-1-[1,2,3,4-¹³C₄]octanesulfonic acid (¹³C₄-PFOS)	5.57	10	503.0	80.0

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
13.	Perfluorooctanesulfonic acid (PFOS)	5.58	10	498.9	80.0
14.	9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)	5.88	5	530.8	350.7
15.	Perfluoro-n-[1,2-¹³C₂]decanoic acid (¹³C₂-PFDA)	6.08	5	514.9	469.9
16.	Perfluorodecanoic acid (PFDA)	6.08	5	512.9	469.0
17.	N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)	6.28	10	572.9	418.8
18.	N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)	6.30	10	569.8	418.8
19.	N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)	6.51	10	588.9	418.8
20.	N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)	6.52	10	583.8	418.8
21.	Perfluoroundecanoic acid (PFUnA)	6.55	5	562.9	518.8
22.	11-chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)	6.77	5	630.7	451.0
23.	Perfluorododecanoic acid (PFDoA)	6.95	5	612.7	568.9
24.	Perfluorotridecanoic acid (PFTrDA)	7.30	5	662.7	618.8
25.	Perfluorotetradecanoic acid (PFTA)	7.60	5	712.7	668.7

Column

Raptor C18 (cat.# 9304A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

96:4 Methanol:water

Conc.:

5-10 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

A:

Water, 5 mM ammonium acetate

B:

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	70	30
8.00	0.4	10	90
8.01	0.4	70	30
10.0	0.4	70	30

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	HPLC

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