Laboratory Fortified Blank (LFB) at Midrange (40 ppt) for EPA 537.1 on Raptor C18

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	2.08	40	299.0	80.0
2.	Perfluoro-n-[1,2-¹³C₂]hexanoic acid (¹³C₂-PFHxA)	3.11	20	315.1	270.1
3.	Perfluorohexanoic acid (PFHxA)	3.11	40	313.2	269.0
4.	Tetrafluoro-2-heptafluoropropoxy-¹³C₃-propanoic acid (¹³C₃-HFPO-DA)	3.47	20	332.1	287.3
5.	Hexafluoropropylene oxide dimer acid (HFPO-DA)	3.47	40	328.9	284.9
6.	Perfluoroheptanoic acid (PFHpA)	4.19	40	363.2	319.2
7.	Perfluorohexanesulfonic acid (PFHxS)	4.33	40	399.2	79.9
8.	4,8-Dioxa-3H-perfluorononanoic acid (ADONA)	4.34	40	376.9	251.0
9.	Perfluoro-[1,2-¹³C₂]octanoic acid (¹³C₂-PFOA)	5.00	20	414.9	370.0
10.	Perfluorooctanoic acid (PFOA)	5.00	40	413.1	369.1
11.	Perfluorononanoic acid (PFNA)	5.64	40	463.1	419.0
12.	Perfluorooctanesulfonic acid (PFOS)	5.66	40	499.2	80.1

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion
13.	Perfluoro-1-[1,2,3,4-¹³C₄]octanesulfonic acid (¹³C₄-PFOS)	5.67	60	503.1	80.2
14.	9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)	5.97	40	531.0	350.9
15.	Perfluoro-n-[1,2-¹³C₂]decanoic acid (¹³C₂-PFDA)	6.18	20	515.2	470.1
16.	Perfluorodecanoic acid (PFDA)	6.18	40	512.9	468.9
17.	N-methyl perfluorooctanesulfonamidoacetic acid (N-MeFOSAA)	6.38	40	570.2	419.0
18.	N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)	6.38	80	573.1	419.1
19.	N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-N-EtFOSAA)	6.61	80	589.2	419.1
20.	N-ethyl perfluorooctanesulfonamidoacetic acid (N-EtFOSAA)	6.62	40	583.8	418.9
21.	Perfluoroundecanoic acid (PFUnA)	6.64	40	563.2	519.1
22.	11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)	6.86	40	630.8	451.1
23.	Perfluorododecanoic acid (PFDoA)	7.05	40	613.1	569.1
24.	Perfluorotridecanoic acid (PFTrDA)	7.40	40	663.0	619.2
25.	Perfluorotetradecanoic acid (PFTA)	7.71	40	713.1	669.0

Column

Raptor C18 (cat.# 9304A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

96:4 Methanol:water

Conc.:

5-20 ng/mL in the final solution after sample preparation (equivalent to 20-80 ppt in laboratory reagent water sample prior to extraction)

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium acetate

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	70	30
8.00	0.4	10	90
8.01	0.4	70	30
10.0	0.4	70	30

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	HPLC
Sample Preparation	The sample was prepared using Resprep S-DVB SPE cartridges (cat.# 28937) mounted on a Resprep vacuum manifold (cat.# 29298-VM) following the procedure in U.S. EPA Method 537.1. While internal standard concentrations varied, all target analytes were fortified at 40 ppt.
Notes	A PFAS delay column (cat.# 27854) was installed before the injector.

Download PDF