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Laboratory Fortified Blank (LFB) at Midrange (40 ppt) for EPA 537.1 on Raptor C18

LC_EV0573
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
1.Perfluorobutanesulfonic acid (PFBS)2.0840299.080.0
2.Perfluoro-n-[1,2-13C2]hexanoic acid (13C2-PFHxA)3.1120315.1270.1
3.Perfluorohexanoic acid (PFHxA)3.1140313.2269.0
4.Tetrafluoro-2-heptafluoropropoxy-13C3-propanoic acid (13C3-HFPO-DA)3.4720332.1287.3
5.Hexafluoropropylene oxide dimer acid (HFPO-DA)3.4740328.9284.9
6.Perfluoroheptanoic acid (PFHpA)4.1940363.2319.2
7.Perfluorohexanesulfonic acid (PFHxS)4.3340399.279.9
8.4,8-Dioxa-3H-perfluorononanoic acid (ADONA)4.3440376.9251.0
9.Perfluoro-[1,2-13C2]octanoic acid (13C2-PFOA)5.0020414.9370.0
10.Perfluorooctanoic acid (PFOA)5.0040413.1369.1
11.Perfluorononanoic acid (PFNA)5.6440463.1419.0
12.Perfluorooctanesulfonic acid (PFOS)5.6640499.280.1
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct Ion
13.Perfluoro-1-[1,2,3,4-13C4]octanesulfonic acid (13C4-PFOS)5.6760503.180.2
14.9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)5.9740531.0350.9
15.Perfluoro-n-[1,2-13C2]decanoic acid (13C2-PFDA)6.1820515.2470.1
16.Perfluorodecanoic acid (PFDA)6.1840512.9468.9
17.N-methyl perfluorooctanesulfonamidoacetic acid (N-MeFOSAA)6.3840570.2419.0
18.N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)6.3880573.1419.1
19.N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-N-EtFOSAA)6.6180589.2419.1
20.N-ethyl perfluorooctanesulfonamidoacetic acid (N-EtFOSAA)6.6240583.8418.9
21.Perfluoroundecanoic acid (PFUnA)6.6440563.2519.1
22.11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)6.8640630.8451.1
23.Perfluorododecanoic acid (PFDoA)7.0540613.1569.1
24.Perfluorotridecanoic acid (PFTrDA)7.4040663.0619.2
25.Perfluorotetradecanoic acid (PFTA)7.7140713.1669.0
ColumnRaptor C18 (cat.# 9304A52)
Dimensions:50 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Temp.:40 °C
Standard/Sample
Diluent:96:4 Methanol:water
Conc.:5-20 ng/mL in the final solution after sample preparation (equivalent to 20-80 ppt in laboratory reagent water sample prior to extraction)
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.47030
8.000.41090
8.010.47030
10.00.47030
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesA PFAS delay column (cat.# 27854) was installed before the injector. The sample was prepared using Resprep S-DVB SPE cartridges (cat.# 28937) mounted on a Resprep vacuum manifold (cat.# 29298-VM) following the procedure in U.S. EPA Method 537.1. While internal standard concentrations varied, all target analytes were fortified at 40 ppt.