Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

PFAS on Force C18 by Direct Injection (EPA Method 8327)

LC_EV0576
PeakstR (min)Conc.
(ng/L)
Precursor IonProduct IonProduct Ion
1.Perfluorobutanoic acid (PFBA)1.8280231.1169.1-
2.Perfluoro-n-[13C4]butanoic acid (M4PFBA)1.8240217.0172.0-
3.Perfluoropentanoic acid (PFPeA)3.0780263.0219.0-
4.Perfluoro-n-[13C5]pentanoic acid (M5PFPeA)3.0740268.0223.0-
5.Perfluorobutane sulfonic acid (PFBS)3.2580299.180.099.0
6.Perfluoro-1-[2,3,4-13C3]butyl sulfonic acid (M3PFBS)3.2540302.080.0-
7.1H, 1H, 2H, 2H-perfluorohexane sulfonic acid (4:2 FTS)3.7980327.1307.180.8
8.1H, 1H, 2H, 2H-perfluoro-1-[1,2-13C2]hexyl sulfonic acid (M2-4:2 FTS)3.7940329.0309.0-
9.Perfluorohexanoic acid (PFHxA)3.8480313.1269.1119.1
10.Perfluoro-n-[1,2,3,4,6-13C5]hexanoic acid (M5PFHxA)3.8440318.0273.0-
11.Perfluoropentane sulfonic acid (PFPeS)3.9280349.180.099.0
12.Perfluoroheptanoic acid (PFHpA)4.3680363.2319.1169.1
13.Perfluoro-n-[1,2,3,4-13C4]heptanoic acid (M4PFHpA)4.3640367.0322.0-
14.Perfluorohexane sulfonic acid (PFHxS)4.4080399.180.099.0
15.Perfluoro-1-[1,2,3-13C3]hexyl sulfonic acid (M3PFHxS)4.4040402.080.0-
16.1H, 1H, 2H, 2H-perfluorooctane sulfonic acid (6:2 FTS)4.7280427.2407.280.7
17.1H, 1H, 2H, 2H-perfluoro-1-[1,2-13C2]octyl sulfonic acid (M2-6:2 FTS)4.7240429.0409.0-
18.Perfluorooctanoic acid (PFOA)4.7580412.9369.0169.0
19.Perfluoro-n-[13C8]octanoic acid (M8PFOA)4.7540421.0376.0-
20.Perfluoroheptane sulfonic acid (PFHpS)4.7580449.280.099.0
21.Perfluorononanoic acid (PFNA)5.0580463.2419.2219.1
22.Perfluoro-n-[13C9]nonanoic acid (M9PFNA)5.0540472.0427.0-
23.Perfluorooctane sulfonic acid (PFOS)5.0580499.280.099.0
24.Perfluoro-1-[13C8]octyl sulfonic acid (M8PFOS)5.0540506.880.0-
25.1H, 1H, 2H, 2H-perfluorodecane sulfonic acid (8:2 FTS)5.2980527.2507.280.8
26.1H, 1H, 2H, 2H-perfluoro-1-[1,2-13C2]decyl sulfonic acid (M2-8:2 FTS)5.2940529.0509.0-
27.Perfluorononane sulfonic acid (PFNS)5.3080549.280.099.0
28.Perfluorodecanoic acid (PFDA)5.3080513.2469.2219.1
29.Perfluoro-n-[1,2,3,4,5,6-13C6]decanoic acid (M6PFDA)5.3040518.9473.9-
30.N-methylperfluoro-1-octanesulfonamidoacetic acid (N-MeFOSAA)5.4380570.2419.2483.2
31.N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)5.4340572.9418.9-
32.Perfluoroundecanoic acid (PFUnA)5.5280563.2519.2269.1
33.Perfluoro-n-[1,2,3,4,5,6,7-13C7]undecanoic acid (M7PFUnA)5.5240569.9524.9-
34.Perfluorodecane sulfonic acid (PFDS)5.5380599.280.099.0
35.N-ethylperfluoro-1-octanesulfonamidoacetic acid (N-EtFOSAA)5.5480584.2419.2483.1
36.N-ethyl-d5-perfluoro-1-catanesulfonamidoacetic acid (d5-N-EtFOSAA)5.5440589.0419.0-
37.Perfluorododecanoic acid (PFDoA)5.7180613.2569.2169.1
38.Perfluoro-n-[1,2,-13C2]dodecanoic acid (M2PFDoA)5.7140614.8569.9-
39.Perfluorooctane sulfonamide (FOSA)5.7580498.278.0-
40.Perfluoro-1-[13C8]octanesulfonamide (M8FOSA)5.7540505.978.0-
41.Perfluorotridecanoic acid (PFTriA)5.8980663.2619.2169.1
42.Perfluorotetradecanoic acid (PFTreA)6.0480713.2669.2169.1
43.Perfluoro-n-[1,2-13C2]tetradecanoic acid (M2PFTreA)6.0440714.8669.7-
ColumnForce C18 (cat.# 9634252)
Dimensions:50 mm x 2.1 mm ID
Particle Size:1.8 µm
Pore Size:100 Å
Temp.:40 °C
Standard/Sample
Diluent:50:50 Water:methanol, 0.1% acetic acid
Conc.:80 ppt (40 ppt for surrogate or isotopically labelled PFAS)
Inj. Vol.:10 µL
Mobile Phase
A:Water, 5 mM ammonium acetate
B:Methanol
Time (min)Flow (mL/min)%A%B
0.000.48020
6.000.4595
6.010.48020
8.000.48020
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentUHPLC
NotesA PFAS delay column (cat.# 27854) was installed before the injector.