PFAS by Direct Injection on Force C18 (3 µm) (EPA Method 8327)

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion	Product Ion
1.	Perfluorobutanoic acid (PFBA)	1.56	80	231.1	169.1	-
2.	Perfluoro-n-[¹³C₄]butanoic acid (M4PFBA)	1.56	40	217.0	172.0	-
3.	Perfluoropentanoic acid (PFPeA)	2.70	80	263.0	219.0	-
4.	Perfluoro-n-[¹³C₅]pentanoic acid (M5PFPeA)	2.70	40	268.0	223.0	-
5.	Perfluorobutane sulfonic acid (PFBS)	2.90	80	299.1	80.0	99.0
6.	Perfluoro-1-[2,3,4-¹³C₃]butyl sulfonic acid (M3PFBS)	2.90	40	302.0	80.0	-
7.	1H, 1H, 2H, 2H-perfluorohexane sulfonic acid (4:2 FTS)	3.43	80	327.1	307.1	80.8
8.	1H, 1H, 2H, 2H-perfluoro-1-[1,2-¹³C₂]hexyl sulfonic acid (M2-4:2 FTS)	3.43	40	329.0	309.0	-
9.	Perfluorohexanoic acid (PFHxA)	3.49	80	313.1	269.1	119.1
10.	Perfluoro-n-[1,2,3,4,6-¹³C₅]hexanoic acid (M5PFHxA)	3.49	40	318.0	273.0	-
11.	Perfluoropentane sulfonic acid (PFPeS)	3.58	80	349.1	80.0	99.0
12.	Perfluoroheptanoic acid (PFHpA)	4.04	80	363.2	319.1	169.1
13.	Perfluoro-n-[1,2,3,4-¹³C₄]heptanoic acid (M4PFHpA)	4.04	40	367.0	322.0	-
14.	Perfluorohexane sulfonic acid (PFHxS)	4.07	80	399.1	80.0	99.0
15.	Perfluoro-1-[1,2,3-¹³C₃]hexyl sulfonic acid (M3PFHxS)	4.07	40	402.0	80.0	-
16.	1H, 1H, 2H, 2H-perfluorooctane sulfonic acid (6:2 FTS)	4.46	80	427.2	407.2	80.7
17.	1H, 1H, 2H, 2H-perfluoro-1-[1,2-¹³C₂]octyl sulfonic acid (M2-6:2 FTS)	4.46	40	429.0	409.0	-
18.	Perfluorooctanoic acid (PFOA)	4.48	80	412.9	369.0	169.0
19.	Perfluoro-n-[¹³C₈]octanoic acid (M8PFOA)	4.48	40	421.0	376.0	-
20.	Perfluoroheptane sulfonic acid (PFHpS)	4.49	80	449.2	80.0	99.0
21.	Perfluorononanoic acid (PFNA)	4.84	80	463.2	419.2	219.1
22.	Perfluoro-n-[¹³C₉]nonanoic acid (M9PFNA)	4.84	40	472.0	427.0	-
23.	Perfluorooctane sulfonic acid (PFOS)	4.84	80	499.2	80.0	99.0
24.	Perfluoro-1-[¹³C₈]octyl sulfonic acid (M8PFOS)	4.84	40	506.8	80.0	-
25.	Perfluorononane sulfonic acid (PFNS)	5.14	80	549.2	80.0	99.0
26.	1H, 1H, 2H, 2H-perfluorodecane sulfonic acid (8:2 FTS)	5.15	80	527.2	507.2	80.8
27.	1H, 1H, 2H, 2H-perfluoro-1-[1,2-¹³C₂]decyl sulfonic acid (M2-8:2 FTS)	5.15	40	529.0	509.0	-
28.	Perfluorodecanoic acid (PFDA)	5.15	80	513.2	469.2	219.1
29.	Perfluoro-n-[1,2,3,4,5,6-¹³C₆]decanoic acid (M6PFDA)	5.15	40	518.9	473.9	-
30.	N-methylperfluoro-1-octanesulfonamidoacetic acid (N-MeFOSAA)	5.28	80	570.2	419.2	483.2
31.	N-methyl-d3-perfluoro-1-octanesulfonamidoacetic acid (d3-N-MeFOSAA)	5.28	40	572.9	418.9	-
32.	Perfluorodecane sulfonic acid (PFDS)	5.40	80	599.2	80.0	99.0
33.	N-ethylperfluoro-1-octanesulfonamidoacetic acid (N-EtFOSAA)	5.42	80	584.2	419.2	483.1
34.	N-ethyl-d5-perfluoro-1-catanesulfonamidoacetic acid (d5-N-EtFOSAA)	5.42	40	589.0	419.0	-
35.	Perfluoroundecanoic acid (PFUnA)	5.42	80	563.2	519.2	269.1
36.	Perfluoro-n-[1,2,3,4,5,6,7-¹³C₇]undecanoic acid (M7PFUnA)	5.42	40	569.9	524.9	-
37.	Perfluorooctane sulfonic acid (FOSA)	5.49	80	498.2	78.0	-
38.	Perfluoro-1-[¹³C₈]octanesulfonamide (M8FOSA)	5.49	40	505.9	78.0	-
39.	Perfluorododecanoic acid (PFDoA)	5.65	80	613.2	569.2	169.1
40.	Perfluoro-n-[1,2,-¹³C₂]dodecanoic acid (M2PFDoA)	5.65	40	614.8	569.9	-
41.	Perfluorotridecanoic acid (PFTriA)	5.86	80	663.2	619.2	169.1
42.	Perfluorotetradecanoic acid (PFTreA)	6.05	80	713.2	669.2	169.1
43.	Perfluoro-n-[1,2-¹³C₂]tetradecanoic acid (M2PFTreA)	6.05	40	714.8	669.7	-

Column

Force C18 (cat.# 9634352)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Temp.:

40 °C

Standard/Sample

Diluent:

50:50 Water:methanol, 0.1% acetic acid

Conc.:

80 ppt (40 ppt for surrogate or isotopically-labelled PFAS)

Inj. Vol.:

10 µL

Mobile Phase

Water, 5 mM ammonium acetate

Methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	80	20
6.00	0.4	5	95
6.50	0.4	5	95
6.51	0.4	80	20
8.50	0.4	80	20

Detector	MS/MS
Ion Mode:	ESI-
Mode:	MRM
Instrument	UHPLC
Notes	A PFAS delay column (cat.# 27854) was installed before the injector.

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