Ultrashort-Chain and Short-Chain PFAS on Raptor Polar X

	Peaks	t_R (min)	Conc. (ng/L)	Precursor Ion	Product Ion	Product Ion
1.	Perfluorobutanesulfonic acid (PFBS)	1.01	400	298.97	79.97	98.89
2.	¹³C₃-Perfluorobutanesulfonic acid (M3PFBS)	1.01	100	301.97	79.97	-
3.	Perfluoropropanesulfonic acid (PFPrS)	1.06	400	248.97	79.91	98.91
4.	Perfluoroethanesulfonic acid (PFEtS)	1.12	400	198.90	79.92	98.91
5.	Trifluoromethanesulfonic acid (TFMS)	1.25	400	148.97	79.93	98.92
6.	Perfluorobutanoic acid (PFBA)	1.93	400	213.03	168.98	-
7.	¹³C₄-Perfluorobutanoic acid (M4PFBA)	1.93	100	217.03	171.98	-
8.	Perfluoropropanoic acid (PFPrA)	2.23	400	162.97	119.02	-
9.	Trifluoroacetic acid (TFA)	3.05	400	113.03	69.01	-

Column

Raptor Polar X (cat.# 9311A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

Reverse osmosis water

Conc.:

100 - 400 ng/L (ppt)

Inj. Vol.:

10 µL

Mobile Phase

Water, 10 mM ammonium formate, 0.1% formic acid

95:5 Acetonitrile:isopropanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.3	15	85
7.00	0.3	15	85

Detector	Waters Xevo TQ-S
Ion Mode:	ESI-
Mode:	MRM
Instrument	Waters ACQUITY UPLC I-Class
Sample Preparation	The standard solution was prepared in reverse osmosis water at 400 ppt. An aliquot of 0.4 mL was transferred to a polypropylene HPLC vial and mixed with 4 µL of internal standard solution (10 ng/mL) containing M3PFBS and M4PFBA.

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