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|Column||Ultra Aqueous C18 (cat.# 9178312)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||3 µm|
|Pore Size:||100 Å|
|Standard/Sample||QuEChERS extract of red bell pepper|
|Nicarbazin (bis-nitrophenol urea) (cat.# 33261)|
|Diluent:||Deionized water:acetonitrile (90:10)|
|Inj. Vol.:||10 µL|
|Mobile Phase||10 mM ammonium acetate in water:10 mM ammonium formate in methanol with gradient program|
|Detector||AB SCIEX API 4000 LC/MS/MS System|
|Sample Preparation||Internal Standard: 100 µL of QuEChERS internal standard mix for GC-MS analysis (cat.# 33267) and 100 µL of QuEChERS internal standard mix for LC-MS/MS analysis (cat.# 33261) were added to each 10 g sample. These internal standard mixes require no dilutions and contain compounds specified in the EN 15662 QuEChERS method. |
Extraction: 10 mL acetonitrile was added to a 10 gram sample of homogenized red bell pepper. After a 1 minute shake, Q-sep European method extraction salts (cat.# 26236) were added followed by another 1 minute shake. The sample was then centrifuged with a Q-sep 3000 centrifuge (cat.# 26230).
dSPE: Add 1 mL extract to QuEChERS dSPE cleanup tube containing 150 mg MgSO4, 25 mg PSA, and 25 mg C18 (cat.# 26216). Shake for 30 seconds and centrifuge with a Q-sep 3000 centrifuge (cat.# 26230).