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Incurred Pesticides in Red Bell Pepper QuEChERS Extract on Ultra Aqueous C18

LC_FF0515
ColumnUltra Aqueous C18 (cat.# 9178312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
SampleQuEChERS extract of red bell pepper
Nicarbazin (bis-nitrophenol urea) (cat.# 33261)
Diluent:Deionized water:acetonitrile (90:10)
Inj. Vol.:10 µL
Mobile Phase10 mM ammonium acetate in water:10 mM ammonium formate in methanol with gradient program
Flow:0.5 mL/min
DetectorAB SCIEX API 4000 LC/MS/MS System
Ion Source:TurboIonSpray®
Ion Mode:ESI+
Mode:MRM
InstrumentAPI LC-MS/MS
NotesInternal Standard: 100 µL of QuEChERS internal standard mix for GC-MS analysis (cat.# 33267) and 100 µL of QuEChERS internal standard mix for LC-MS/MS analysis (cat.# 33261) were added to each 10 g sample. These internal standard mixes require no dilutions and contain compounds specified in the EN 15662 QuEChERS method.

Extraction: 10 mL acetonitrile was added to a 10 gram sample of homogenized red bell pepper. After a 1 minute shake, Q-sep European method extraction salts (cat.# 26236) were added followed by another 1 minute shake. The sample was then centrifuged with a Q-sep 3000 centrifuge (cat.# 26230).

dSPE: Add 1 mL extract to QuEChERS dSPE cleanup tube containing 150 mg MgSO4, 25 mg PSA, and 25 mg C18 (cat.# 26216). Shake for 30 seconds and centrifuge with Q-sep 3000 centrifuge (cat.# 26230).