LC Multiresidue Pesticide Standard #5 on Ultra Aqueous C18 by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion (m/z)	Product Ion 1 (m/z)	Product Ion 2 (m/z)
1.	Thiamethoxam	4.2	292.0	211.0	181.0
2.	Clothianidin	4.4	250.0	169.0	132.0
3.	Thiabendazole	4.7	202.1	175.1	131.2
4.	Mesotrione	5.0	340.2	228.2	104.1
5.	Thiacloprid	5.1	253.0	126.0	99.0
6.	Sulfentrazone	5.3	387.0	307.1	146.0
7.	Tricyclazole	5.3	190.0	163.0	136.0
8.	Metribuzin	5.4	215.1	187.1	84.1
9.	Carboxin	5.6	236.1	143.0	87.0
10.	Thiofanox	5.8	219.2	57.2	61.1
11.	Simetryn	5.9	214.0	124.0	144.0
12.	Ethofumesate	6.5	287.1	259.1	121.1
13.	Ethiprole	6.7	397.3	255.2	350.9
14.	Methoprotryne	6.7	272.2	198.0	240.2
15.	Fenamidone	6.8	312.1	92.0	236.1

	Peaks	t_R (min)	Precursor Ion (m/z)	Product Ion 1 (m/z)	Product Ion 2 (m/z)
16.	Clethodim isomer 1	7.0	360.1	164.0	268.1
17.	Acibenzolar-S-methyl	7.3	211.0	136.2	91.2
18.	Prometryne	7.4	242.2	158.1	200.1
19.	Terbutryn	7.6	242.1	186.1	68.1
20.	Flufenacet	7.7	364.1	152.1	194.2
21.	Fipronil	7.8	437.1	368.0	290.0
22.	Mefenacet	7.8	299.0	148.1	120.1
23.	Bupirimate	8.0	317.0	166.1	108.0
24.	Cyazofamid	8.0	325.2	108.0	261.2
25.	Flubendiamide	8.4	683.1	408.0	274.1
26.	Prothioconazole	8.7	344.1	188.9	125.1
27.	Clethodim isomer 2	9.9	360.1	164.0	268.1
28.	Buprofezin	10.2	306.2	201.1	116.2
29.	Hexythiazox	10.7	353.1	168.0	228.0
30.	Propargite	10.8	368.2	231.1	175.1
31.	Pyridaben	11.3	365.0	147.0	309.0

Isomers are numbered sequentially based on elution order. To download this retention table in XLS format, visit www.restek.com/LC-multiresidue

Column

Ultra Aqueous C18 (cat.# 9178312)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Temp.:

50 °C

Standard/Sample

LC multiresidue pesticide standard #5 (cat.# 31976)

Diluent:

Water

Conc.:

50 ng/mL

Inj. Vol.:

20 µL

Mobile Phase

Water + 4 mM ammonium formate + 0.1% formic acid

Methanol + 4 mM ammonium formate + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	90	10
1.50	0.5	90	10
4.00	0.5	40	60
8.00	0.5	30	70
11.00	0.5	0	100
12.00	0.5	0	100
12.01	0.5	90	10
15.00	0.5	90	10

Max Pressure:

255 bar

Detector	AB SCIEX API 4000 LC-MS/MS
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Ion Spray Voltage:	5.5 kV
Curtain Gas:	30 psi (206.8 kPa)
Gas 1:	40 psi (275.8 kPa)
Gas 2:	45 psi (310.3 kPa)
CAD:	10 psi (68.9 kPa)
Source Temp.:	350 °C
Mode:	Scheduled MRM
MRM Detection Window:	60 sec
Target Scan Time:	0.33 sec
Instrument	API LC-MS/MS
Notes	Q1/Q3 Resolution: Unit Autosampler Temp.: 5 °C

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