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Peaks | tR (min) | Precursor Ion | Product Ion | |
---|---|---|---|---|
1. | Retinol | 1.46 | 269.4 | 93.3 |
2. | Vitamin D2 | 3.52 | 397.5 | 379.6 |
3. | Vitamin D3 | 3.58 | 385.5 | 367.5 |
4. | γ-Tocopherol | 3.78 | 417.6 | 151.4 |
5. | α-Tocopherol | 4.12 | 431.7 | 165.4 |
6. | Vitamin K1 | 5.23 | 451.4 | 187.3 |
Column | Raptor ARC-18 (cat.# 9314A12) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | Methanol | ||||||||||||||||||||||||
Conc.: | 100 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | 0.1% Formic acid + 5 mM ammonium formate in water | ||||||||||||||||||||||||
B: | 0.1% Formic acid + 5 mM ammonium formate in methanol | ||||||||||||||||||||||||
| |||||||||||||||||||||||||
Max Pressure: | 190 bar |
Detector | ABSCIEX API 4000 |
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Ion Source: | TurboIonSpray® |
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | Shimadzu UFLCXR |
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