Fat-Soluble Vitamins on Raptor ARC-18 by LC-MS/MS

LC_FF0537

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Retinol	1.46	269.4	93.3
2.	Vitamin D2	3.52	397.5	379.6
3.	Vitamin D3	3.58	385.5	367.5
4.	γ-Tocopherol	3.78	417.6	151.4
5.	α-Tocopherol	4.12	431.7	165.4
6.	Vitamin K1	5.23	451.4	187.3

Column

Raptor ARC-18 (cat.# 9314A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

40 °C

Standard/Sample

Diluent:

Methanol

Conc.:

100 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Formic acid + 5 mM ammonium formate in water

B:

0.1% Formic acid + 5 mM ammonium formate in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	10	90
4.0	0.5	0	100
5.0	0.5	0	100
5.01	0.5	10	90
7.0	0.5	10	90

Max Pressure:

190 bar

Detector	ABSCIEX API 4000
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Mode:	MRM
Instrument	Shimadzu UFLCXR

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