Anthocyanidins on Raptor ARC-18 5 μm by HPLC

LC_FF0556

	Peaks	t_R (min)
1.	Delphinidin	0.724
2.	Cyanidin	0.992
3.	Pelargonidin	1.288
4.	Malvidin	1.442

Column

Raptor ARC-18 (cat.# 931455E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

5 µm

Temp.:

40 °C

Standard/Sample

Diluent:

0.1% Trifluoroacetic acid in 50:50 acetonitrile:water

Conc.:

30 µg/mL

Inj. Vol.:

5 µL

Mobile Phase

A:

0.1% Trifluoroacetic acid in water

B:

0.1% Trifluoroacetic acid in methanol

Time (min)	Flow (mL/min)	%B
0.00	1.0	35
2.00	1.0	55
2.01	1.0	35
4.00	1.0	35

Detector	PDA @ 530 nm
Instrument	HPLC

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