Water Soluble Vitamins by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Thiamine (B1)	1.26	50	265.0	122.1	144.1
2.	Pyridoxine (B6)	1.35	20	170.1	152.1	134.1
3.	Niacin (B3)	1.84	200	123.1	80.1	78.1
4.	Pantothenic acid (B5)	1.94	200	220.0	90.1	72.1
5.	Folic acid (B9)	2.78	500	442.1	295.0	176.3
6.	Cyanocobalamin (B12)	2.92	500	678.3	147.2	359.0
7.	Biotin (B7)	2.93	100	245.0	227.1	97.1
8.	Riboflavin (B2)	3.12	200	377.0	243.1	172.1

Column

Ultra Aqueous C18 (cat.# 9178312)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Temp.:

40 °C

Standard/Sample

Diluent:

Water

Inj. Vol.:

2 µL

Mobile Phase

Water + 5 mM ammonium formate + 0.1% formic acid

Methanol + 5 mM ammonium formate + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	100	0
3.50	0.6	35	65
3.51	0.6	100	0
6.00	0.6	100	0

Download PDF