Artificial Sweeteners on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Acesulfame-K	0.83	100	162.04	82.07	77.97
2.	Cyclamate	1.07	100	178.14	79.99	96.00
3.	Saccharin	1.17	100	182.02	106.01	61.97
4.	Sucralose	1.45	100	441.23	395.17	359.21
5.	Aspartame	1.50	100	293.27	261.19	200.12
6.	Alitame	1.58	100	330.30	312.23	295.19
7.	Dulcin	1.83	100	181.13	138.60	107.64
8.	Neohesperidin dihydrochalcone	2.17	100	611.40	303.21	166.13
9.	Stevioside	2.31	100	803.58	641.52	479.45
10.	Neotame	2.45	100	377.34	200.15	345.30
11.	Glycyrrhizic acid	2.83	100	821.67	351.19	113.07

Column

Raptor Biphenyl (cat.# 9309252)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

1.8 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

Water

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

Water, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.3	95	5
3.00	0.3	45	55
3.01	0.3	95	5
4.00	0.3	95	5

Detector	MS/MS
Mode:	MRM
Instrument	UHPLC
Notes	Dulcin was analyzed in ESI+ mode. All other compounds were analyzed in ESI- mode.

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