Pyrrolizidine Alkaloids on Raptor ARC-18 by LC-MS/MS

LC_FF0573

	Peaks	t_R (min)	Precursor	Product 1	Product 2	Product 3
1.	Monocrotaline	0.677	326.20	120.3	121.30	94.25
2.	Incidine	1.111	300.20	94.15	138.20	156.36
3.	Heliotrine	1.763	314.30	138.20	156.20	120.20
4.	Heliotrine N-oxide	1.895	330.25	172.25	138.20
5.	Senecionine	2.240	336.20	120.30	138.30
6.	Senecionine N-oxide	2.410	352.35	94.20	120.30	118.25
7.	Echimidine	2.524	398.30	120.25	220.15
8.	Echimidine N-oxide	2.528	414.25	396.30	352.20	254.25
9.	Lasiocarpine	3.028	412.20	120.20	220.15	336.25
10.	Lasiocarpine N-oxide	3.260	428.25	254.25	352.25	120.30

Column

Raptor ARC-18 (cat.# 9314A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

25 °C

Standard/Sample

Diluent:

0.1% Formic acid in water

Conc.:

10 ng/mL

Inj. Vol.:

1 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	95	5
3.5	0.5	65	35
3.51	0.5	95	5
4.5	0.5	95	5

Detector	MS/MS
Ion Mode:	ESI+
Instrument	UHPLC

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