Analysis of 21 Cannabinoids on Raptor ARC-18 by LC-MS

	Peaks	t_R (min)	Conc. (ng/mL)	SIM	ESI
1.	Cannabidivarin (CBDV)	1.44	500	287.0	+
2.	Cannabidivarinic acid (CBDVA)	1.56	500	331.0	+
3.	Cannabidiol (CBD)	2.14	500	315.0	+
4.	Cannabigerol (CBG)	2.15	500	317.0	+
5.	Cannabidiolic acid (CBDA)	2.26	500	357.0	-
6.	Tetrahydrocannabivarin (THCV)	2.44	500	287.0	+
7.	Cannabigerolic acid (CBGA)	2.70	500	359.0	-
8.	Cannabichromevarin (CBCV)	3.15	500	287.0	+
9.	Cannabinol (CBN)	3.43	500	311.0	+
10.	Cannabidiphorol (CBDP)	3.62	500	343.5	+

	Peaks	t_R (min)	Conc. (ng/mL)	SIM	ESI
11.	Tetrahydrocannabivarinic acid (THCVA)	3.97	500	331.0	+
12.	Δ9-Tetrahydrocannabinol (Δ9-THC)	4.25	500	315.0	+
13.	Δ8-Tetrahydrocannabinol (Δ8-THC)	4.55	500	315.0	+
14.	Cannabinolic acid (CBNA)	4.91	500	353.0	-
15.	Cannabicyclol (CBL)	4.94	500	315.0	+
16.	Cannabichromene (CBC)	5.73	500	315.0	+
17.	Tetrahydrocannabinolic acid A (THCA-A)	7.52	500	357.0	-
18.	Cannabicitran (CBT)	7.55	500	315.0	+
19.	Tetrahydrocannabiphorol (THCP)	8.28	500	343.5	+
20.	Cannabichromenic acid (CBCA)	8.31	500	357.0	-
21.	Cannabicyclolic acid (CBLA)	8.79	500	357.0	-

Column

Raptor ARC-18 (cat.# 9314A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

30 °C

Standard/Sample

Cannabidivarinic acid (cat.# 34134)

Cannabidivarin (cat.# 34123)

Cannabidiolic acid (cat.# 34094)

Cannabigerolic acid (cat.# 34135)

Cannabigerol (cat.# 34091)

Cannabidiol (cat.# 34011)

Tetrahydrocannabivarin (cat.# 34100)

Cannabinol (cat.# 34010)

d9-Tetrahydrocannabinol (cat.# 34067)

d8-Tetrahydrocannabinol (cat.# 34090)

Cannabicyclol (cat.# 34130)

Cannabichromene (cat.# 34092)

d9-Tetrahydrocannabinolic acid A (cat.# 34111)

Diluent:

Water:acetonitrile (20:80)

Conc.:

500 ppb ng/mL

Inj. Vol.:

2 µL

Mobile Phase

Water, 0.1% formic acid, 12 mM ammonium formate

Acetonitrile:methanol (50:50), 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	20	80
9	0.5	20	80

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