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Peaks | tR (min) | |
---|---|---|
1. | Cannabidiolic acid (CBDA) | 2.433 |
2. | Cannabigerolic acid (CBGA) | 2.566 |
3. | Cannabigerol (CBG) | 2.726 |
Column | Raptor ARC-18 (cat.# 9314A65) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 150 mm x 4.6 mm ID | ||||||||||||
Particle Size: | 2.7 µm | ||||||||||||
Pore Size: | 90 Å | ||||||||||||
Guard Column: | Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250) | ||||||||||||
Temp.: | 30 °C | ||||||||||||
Standard/Sample | |||||||||||||
Diluent: | 25:75 Water:acetonitrile | ||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||
Mobile Phase | |||||||||||||
A: | Water, 5 mM ammonium formate, 0.1% formic acid | ||||||||||||
B: | Acetonitrile, 0.1% formic acid | ||||||||||||
|
Detector | UV/VIS @ 228 nm |
---|---|
Instrument | HPLC |
Sample Preparation | 500 mg of ground CBG hemp flower was weighed into a 50 mL centrifuge tube. 10 mL of methanol:water (80:20) was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 4200 rpm. The supernatant was diluted 20-fold in water:acetonitrile (25:75), vortexed briefly, and filtered using a 0.2 µm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis. |
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