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Flavonoids on Raptor Biphenyl by LC-MS/MS

LC_FF0586
PeakstR (min)Conc.
(µg/g)
Precursor IonProduct Ion 1Product Ion 2Product Ion 3Polarity
1.Orientin1.4690.1449329.1299.1431+
2.Vitexin1.8270.1433313.1283415.1+
3.Rutin1.8951609.1300.1301271-
4.Quercetin-3-D-glycopyranoside2.010.1463.1300301271-
5.Isovitexin2.0240.1433.4283313.1337+
6.Luteolin-7-O-glucuronide2.2210.1463287.1153.2135.1+
7.Fisetin2.4351285135121163-
8.Apigenin-7-O-glucuronide2.6640.1447271.1153.2119.1+
9.Quercetin2.7531301151179107-
10.Luteolin2.9191285133151175-
11.Kaempferol3.137128592.9239185-
12.Apigenin3.2660.127115311969.1+
13.Silymarin3.3221481.1301125.1152-
14.Baicalin3.3630.1447.1271.1123.1253.1+
15.Chrysin4.1411253143.263.1209-
16.Wogonin4.2200.1285270.1151.2179+
17.Cannflavin B4.3210.1369.1313298165+
18.Cannflavin A4.6200.1437.1313.1165298.1+
19.B-sitosterol5.4721397.4161.1135.1147.2+
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:30 °C
Sample
Diluent:Water:methanol (60:40)
Inj. Vol.:2 µL
Mobile Phase
A:Water, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.56040
4.000.50100
5.500.50100
5.510.56040
7.000.56040
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI+/ESI-
Mode:MRM
InstrumentUHPLC