Flavonoids on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (µg/g)	Precursor Ion	Product Ion 1	Product Ion 2	Product Ion 3	Polarity
1.	Orientin	1.469	0.1	449	329.1	299.1	431	+
2.	Vitexin	1.827	0.1	433	313.1	283	415.1	+
3.	Rutin	1.895	1	609.1	300.1	301	271	-
4.	Quercetin-3-D-glycopyranoside	2.01	0.1	463.1	300	301	271	-
5.	Isovitexin	2.024	0.1	433.4	283	313.1	337	+
6.	Luteolin-7-O-glucuronide	2.221	0.1	463	287.1	153.2	135.1	+
7.	Fisetin	2.435	1	285	135	121	163	-
8.	Apigenin-7-O-glucuronide	2.664	0.1	447	271.1	153.2	119.1	+
9.	Quercetin	2.753	1	301	151	179	107	-
10.	Luteolin	2.919	1	285	133	151	175	-
11.	Kaempferol	3.137	1	285	92.9	239	185	-
12.	Apigenin	3.266	0.1	271	153	119	69.1	+
13.	Silymarin	3.322	1	481.1	301	125.1	152	-
14.	Baicalin	3.363	0.1	447.1	271.1	123.1	253.1	+
15.	Chrysin	4.141	1	253	143.2	63.1	209	-
16.	Wogonin	4.220	0.1	285	270.1	151.2	179	+
17.	Cannflavin B	4.321	0.1	369.1	313	298	165	+
18.	Cannflavin A	4.620	0.1	437.1	313.1	165	298.1	+
19.	B-sitosterol	5.472	1	397.4	161.1	135.1	147.2	+

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

Water:methanol (60:40)

Inj. Vol.:

2 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	60	40
4.00	0.5	0	100
5.50	0.5	0	100
5.51	0.5	60	40
7.00	0.5	60	40

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