Flavonoids in CBG Hemp Flower on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor	Product Ion 1	Product Ion 2	Product Ion 3	Polarity
1.	Orientin	1.469	449	329.1	299.1	431	+
2.	Rutin	1.895	609.1	300.1	301	271	-
3.	Quercetin-3-D-glycopyranoside	2.010	463.1	300	301	271	-
4.	Luteolin-7-O-glucuronide	2.221	463	287.1	153.2	135.1	+
5.	Apigenin-7-O-glucuronide	2.664	447	271.1	153.2	119.1	+
6.	Luteolin	2.919	285	133	151	175	-
7.	Apigenin	3.266	271	153	119	69.1	+
8.	Cannflavin B	4.321	369.1	313	298	165	+
9.	Cannflavin A	4.620	437.1	313.1	165	298.1	+
10.	B-sitosterol	5.472	397.4	161.1	135.1	147.2	+

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

30 °C

Standard/Sample

Diluent:

Water:methanol (60:40)

Inj. Vol.:

2 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	60	40
4.00	0.5	0	100
5.50	0.5	0	100
5.51	0.5	60	40
7.00	0.5	60	40

Detector	MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+/ESI-
Mode:	MRM
Instrument	UHPLC
Sample Preparation	500 mg of ground CBG hemp flower was weighed into a 50 mL centrifuge tube. 10 mL of methanol:water (80:20) was added prior to vortexing (5 seconds) and sonicating (15 minutes). The sample was then centrifuged for 5 minutes at 4200 rpm. The supernatant was diluted 50-fold in water:methanol (60:40), vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.

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