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Peaks | tR (min) | Precursor | Product Ion 1 | Product Ion 2 | Product Ion 3 | Polarity | |
---|---|---|---|---|---|---|---|
1. | Orientin | 1.469 | 449 | 329.1 | 299.1 | 431 | + |
2. | Rutin | 1.895 | 609.1 | 300.1 | 301 | 271 | - |
3. | Quercetin-3-D-glycopyranoside | 2.010 | 463.1 | 300 | 301 | 271 | - |
4. | Luteolin-7-O-glucuronide | 2.221 | 463 | 287.1 | 153.2 | 135.1 | + |
5. | Apigenin-7-O-glucuronide | 2.664 | 447 | 271.1 | 153.2 | 119.1 | + |
6. | Luteolin | 2.919 | 285 | 133 | 151 | 175 | - |
7. | Apigenin | 3.266 | 271 | 153 | 119 | 69.1 | + |
8. | Cannflavin B | 4.321 | 369.1 | 313 | 298 | 165 | + |
9. | Cannflavin A | 4.620 | 437.1 | 313.1 | 165 | 298.1 | + |
10. | B-sitosterol | 5.472 | 397.4 | 161.1 | 135.1 | 147.2 | + |
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | Water:methanol (60:40) | ||||||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water, 0.1% formic acid | ||||||||||||||||||||||||
B: | Methanol, 0.1% formic acid | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Source: | Electrospray |
Ion Mode: | ESI+/ESI- |
Mode: | MRM |
Instrument | UHPLC |
Sample Preparation | 500 mg of ground CBG hemp flower was weighed into a 50 mL centrifuge tube. 10 mL of methanol:water (80:20) was added prior to vortexing (5 seconds) and sonicating (15 minutes). The sample was then centrifuged for 5 minutes at 4200 rpm. The supernatant was diluted 50-fold in water:methanol (60:40), vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis. |
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