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Flavonoids in CBD Hemp Flower on Raptor Biphenyl by LC-MS/MS

LC_FF0588
PeakstR (min)Precursor IonProduct Ion 1Product Ion 2Product Ion 3Polarity
1.Orientin1.469449329.1299.1431+
2.Vitexin1.827433313.1283415.1+
3.Rutin1.895609.1300.1301271-
4.Quercetin-3-D-glycopyranoside2.01463.1300301271-
5.Luteolin-7-O-glucuronide2.221463287.1153.2135.1+
6.Apigenin-7-O-glucuronide2.664447271.1153.2119.1+
7.Quercetin2.753301151179107-
8.Luteolin2.919285133151175-
9.Apigenin3.26627115311969.1+
10.Cannflavin B4.321369.1313298165+
11.Cannflavin A4.62437.1313.1165298.1+
12.B-sitosterol5.472397.4161.1135.1147.2+
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:30 °C
Sample
Diluent:Water:methanol (60:40)
Inj. Vol.:2 µL
Mobile Phase
A:Water, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.56040
4.000.50100
5.500.50100
5.510.56040
7.000.56040
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI+/ESI-
InstrumentUHPLC
Notes500 mg of ground CBD hemp flower was weighed into a 50 mL centrifuge tube. 10 mL of methanol:water (80:20) was added prior to vortexing (5 seconds) and sonicating (15 minutes). The sample was then centrifuged for 5 minutes at 4200 rpm. The supernatant was diluted 50-fold in water:methanol (60:40), vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.