CBG Isolate on Raptor ARC-18 (2.7 μm) by HPLC-UV

	Peaks	t_R (min)
1.	Cannabigerol (CBG)	2.897

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Diluent:

25:75 Water:acetonitrle

Conc.:

Endogenous concentration

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
5	1.5	25	75

Detector	UV/Vis @ 228 nm
Sample Preparation	0.5 gram of CBG isolate was weighed into a 50 mL centrifuge tube. 25 mL of IPA was added prior to vortexing (30 seconds) and sonicating (15 minutes). 0.5 mL of sample was aliquoted to a 4 mL vial and diluted 8-fold with 25:75 water:acetonitrile and vortexed briefly. 100 µL of sample was aliquoted to an autosampler vial, diluted 10-fold in 25:75 water:acetonitrile, and vortexed briefly prior to analysis.

Detector

UV/Vis @ 228 nm

Sample Preparation

0.5 gram of CBG isolate was weighed into a 50 mL centrifuge tube. 25 mL of IPA was added prior to vortexing (30 seconds) and sonicating (15 minutes). 0.5 mL of sample was aliquoted to a 4 mL vial and diluted 8-fold with 25:75 water:acetonitrile and vortexed briefly. 100 µL of sample was aliquoted to an autosampler vial, diluted 10-fold in 25:75 water:acetonitrile, and vortexed briefly prior to analysis.

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