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|Column||Raptor ARC-18 (cat.# 9314A65)|
|Dimensions:||150 mm x 4.6 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)|
|Inj. Vol.:||5 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Sample Preparation||0.5 gram of CBG isolate was weighed into a 50 mL centrifuge tube. 25 mL of IPA was added prior to vortexing (30 seconds) and sonicating (15 minutes). 0.5 mL of sample was aliquoted to a 4 mL vial and diluted 8-fold with 25:75 water:acetonitrile and vortexed briefly. 100 µL of sample was aliquoted to an autosampler vial, diluted 10-fold in 25:75 water:acetonitrile, and vortexed briefly prior to analysis.|
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