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CBN Isolate on Raptor ARC-18 (2.7 μm) by HPLC-UV

PeakstR (min)
1.Cannabinol (CBN)4.587
ColumnRaptor ARC-18 (cat.# 9314A65)
Dimensions:150 mm x 4.6 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)
Temp.:30 °C
Diluent:25:75 Water:acetonitrle
Conc.:Endogenous concentration
Inj. Vol.:5 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/Vis @ 228 nm
Sample Preparation0.5 gram of CBN isolate was weighed into a 50 mL centrifuge tube. 25 mL of IPA was added prior to vortexing (30 seconds) and sonicating (15 minutes). 0.5 mL of sample was aliquoted to a 4 mL vial and diluted 8-fold with 25:75 water:acetonitrile and vortexed briefly. 100 uL of sample was aliquoted to an autosampler vial, diluted 10-fold in 25:75 water:acetonitrile, and vortexed briefly prior to analysis.