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CBD-Infused Beverage on Raptor ARC-18 (2.7 μm) by HPLC-UV

LC_FF0611
PeakstR (min)
1.Cannabidiol (CBD)3.259
ColumnRaptor ARC-18 (cat.# 9314A65)
Dimensions:150 mm x 4.6 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)
Temp.:30 °C
Standard/Sample
Conc.:Endogenous concentration
Inj. Vol.:5 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B: Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.001.52575
41.52575
DetectorUV/VIS @ 228 nm
Sample PreparationBeverage was sonicated for 30 min to remove carbonation. 10 mL of beverage was added to a 50 mL centrifuge tube. 10 mL of acetonitrile was added along with an EN Q-sep QuEChERS salt packet (cat.# 25849), and the sample was shaken by hand for 1 minute and then vortexed for 30 seconds. The sample was transferred to a shaker table and shaken for 10 minutes followed by centrifugation at 4200 rpm for 10 minutes. An aliquot was transferred to an autosampler vial prior to analysis.