UHPLC Potency for 18 Cannabinoids Including Delta-10-THC Epimers and Exo-THC

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	1.039
2.	Cannabidivarin (CBDV)	1.160
3.	Cannabidiolic acid (CBDA)	1.449
4.	Cannabigerolic acid (CBGA)	1.550
5.	Cannabigerol (CBG)	1.638
6.	Cannabidiol (CBD)	1.719
7.	Tetrahydrocannabivarin (THCV)	1.870
8.	Tetrahydrocannabivarinic acid (THCVA)	2.361
9.	Cannabinol (CBN)	2.557

	Peaks	t_R (min)
10.	Cannabinolic acid (CBNA)	2.923
11.	Exo-tetrahydrocannabinol (exo-THC)	3.103
12.	d9-Tetrahydrocannabinol (9-THC)	3.201
13.	d8-Tetrahydrocannabinol (8-THC )	3.310
14.	(6aR,9S)-delta-10-Tetrahydrocannabinol (9S-10-THC)	3.719
15.	(6aR,9R)-delta-10-Tetrahydrocannabinol (9R-10-THC)	3.916
16.	Cannabichromene (CBC)	4.042
17.	δ-9-Tetrahydrocannabinolic acid-A (THCA)	4.369
18.	Cannabichromenic acid (CBCA)	4.838

Column

Raptor ARC-18 (cat.# 931421E)

Dimensions:

100 mm x 3 mm ID

Particle Size:

1.8 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge 5 mm, 3.0 mm ID, UHPLC µm (cat.# 9314U0253)

Temp.:

30 °C

Standard/Sample

Cannabinoids acids 7 standard (cat.# 34144)

Cannabidivarin (CBDV) (cat.# 34123)

Cannabigerol (CBG) (cat.# 34091)

Cannabidiol (CBD) (cat.# 34011)

Tetrahydrocannabivarin (THCV) (cat.# 34100)

Cannabinol (CBN) (cat.# 34010)

d9-Tetrahydrocannabinol (d9-THC) (cat.# 34067)

d8-Tetrahydrocannabinol (d8-THC) (cat.# 34090)

Cannabichromene (CBC) (cat.# 34092)

Compounds not present in these mixes were obtained separately.

Diluent:

25:75 Water:acetonitrile

Conc.:

50 µg/mL

Inj. Vol.:

1 µL

Mobile Phase

25:75 Water:acetonitrile, 2.5 mM ammonium formate, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	100	0
2.50	0.8	90	10
4.50	0.8	90	10
4.51	0.8	100	0
6.00	0.8	100	0

Detector	UV/VIS @ 228 nm
Instrument	UHPLC
Sample Preparation	Standards were prepared in a 2 mL screw-thread vial (cat.# 21143) and capped with a short-cap, screw-vial closure (cat.#24498).
Notes	Flow cell = 500 nL

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