Trifluoroacetic Acid (TFA) on Raptor Polar X

	Peaks	t_R (min)	Precursor Ion	Product Ion
1.	Trifluoroacetic acid (TFA)	1.29	113.00	69.05

Column

Raptor Polar X (cat.# 9311A32)

Dimensions:

30 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Diluent:

15:85 Mobile phase A:mobile phase B

Conc.:

200 ng/L

Inj. Vol.:

3 µL

Mobile Phase

Water, 10 mM ammonium formate, 0.05% formic acid

80:20 Acetonitrile:methanol, 0.05% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	15	85
2	0.5	15	85

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI-
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	The sample was aliquoted into a 2.0 mL, 9 mm screw-thread polypropylene vial (cat.# 23242) and capped with a 9 mm solid-top polyethylene cap (cat.# 23244).

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