The Resolution of 9-THC, 8-THC, hydroxy- and carboxy- metabolites on Raptor FluoroPhenyl

	Peaks	t_R (min)	Precursor	Product 1	Product 2
1.	11-OH-Δ8-THC	3.65	331.3	313.3	193.2
2.	11-OH-Δ9-THC	3.94	331.3	313.3	193.2
3.	Δ8-THC-COOH	4.07	345.0	327.2	299.2
4.	Δ9-THC-COOH	4.77	345.0	327.2	299.2
5.	Δ8-THC	10.81	315.0	193.2	123.2
6.	Δ9-THC	11.41	315.0	193.2	123.2

Column

Raptor FluoroPhenyl (cat.# 9319A1E)

Dimensions:

100 mm x 3.0 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

30 °C

Standard/Sample

Δ9-Tetrahydrocannabinol (Δ9-THC) (cat.# 34067)

(±)11-nor-9-carboxy-Δ-9-THC (Δ9-THC-COOH) (cat.# 34068)

Δ8-Tetrahydrocannabinol (Δ8-THC) (cat.# 34090)

Other compounds obtained separately.

Diluent:

37:63 Water:methanol containing 0.1% formic acid

Conc.:

100 ng/mL

Inj. Vol.:

3 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.9	37	63
12.00	0.9	37	63

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	Standards were aliquoted into 2 mL, screw-thread vials (cat.# 21143) and capped with short-cap, screw-vial closures (cat.# 24498).

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