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Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
1. | Tildipirosin | 1.01 | 200 | 734.59 | 561.45 | 204.15 |
2. | Lincomycin | 1.11 | 50 | 407.32 | 359.23 | 389.28 |
3. | Tulathromycin A | 1.19 | 100 | 806.65 | 577.42 | 420.31 |
4. | Spiramycin | 1.41 | 200 | 843.64 | 540.36 | 699.48 |
5. | Pirlymycin | 1.49 | 20 | 411.32 | 363.18 | 327.21 |
Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
6. | Gamithromycin | 1.50 | 100 | 777.63 | 619.52 | 601.45 |
7. | Tilmicosin | 1.58 | 100 | 869.72 | 696.50 | 522.42 |
8. | Erythromycin | 1.80 | 25 | 734.64 | 576.40 | 558.38 |
9. | Tylosin | 1.87 | 100 | 916.62 | 772.49 | 598.36 |
10. | Tylvalosin | 2.30 | 50 | 1042.71 | 814.46 | 640.39 |
11. | Virginiamycin M1 | 2.45 | 50 | 526.43 | 508.31 | 355.10 |
Column | Raptor C18 (cat.# 9304A12) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9304A0252) | ||||||||||||||||||||
Temp.: | 40 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | Water | ||||||||||||||||||||
Conc.: | 20–200 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||
B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
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