Ionophore Antibiotics on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion	Product Ion
1.	Lasalocid A	1.92	100	613.42	377.28	595.40
2.	Monensin	2.12	100	693.50	675.44	461.30
3.	Salinomycin	2.19	100	773.57	431.24	531.39
4.	Maduramicin	2.30	100	939.65	877.58	473.31
5.	Narasin	2.58	100	787.59	431.27	531.35

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

40 °C

Standard/Sample

Diluent:

Water:methanol (10:90)

Conc.:

100 ng/mL

Inj. Vol.:

2 µL

Mobile Phase

0.5% Formic acid in water

0.5% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	35	65
3.00	0.5	25	75
3.01	0.5	35	65
5.00	0.5	35	65

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