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Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|---|
1. | Desacetyl cephapirin | 0.70 | 150 | 382.03 | 111.92 | 124.21 |
2. | Sulfanilamide | 0.85 | 200 | 172.98 | 93.07 | 75.23 |
3. | Amoxicillin | 0.92 | 100 | 366.24 | 349.10 | 208.07 |
4. | Cephapirin | 1.36 | 50 | 424.17 | 292.08 | 124.14 |
5. | Tildipirosin | 1.38 | 200 | 734.59 | 561.45 | 204.15 |
6. | Desfuroyl ceftiofur cysteine disulfide | 1.40 | 300 | 549.16 | 183.02 | 126.00 |
7. | Lincomycin | 1.50 | 50 | 407.32 | 359.23 | 389.28 |
8. | Sulfadiazine | 1.57 | 20 | 251.18 | 156.04 | 92.08 |
9. | Cefquinome | 1.73 | 200 | 529.19 | 134.10 | 125.12 |
10. | Ampicillin | 1.78 | 50 | 350.19 | 106.07 | 160.06 |
11. | Sulfathiazole | 1.79 | 10 | 256.16 | 156.03 | 92.08 |
12. | Marbofloxacin | 1.81 | 10 | 363.20 | 72.11 | 320.10 |
13. | Cefalexin | 1.82 | 100 | 348.10 | 158.05 | 174.05 |
14. | Sulfapyridine | 1.86 | 10 | 250.13 | 156.10 | 92.08 |
15. | Norfloxacin | 1.96 | 20 | 320.23 | 276.20 | 233.13 |
16. | Ofloxacin | 1.98 | 10 | 362.21 | 318.20 | 261.15 |
17. | Sulfamerazine | 2.00 | 20 | 265.08 | 156.03 | 92.08 |
18. | Cefalonium | 2.01 | 100 | 459.16 | 337.03 | 123.10 |
19. | Oxytetracycline | 2.02 | 25 | 461.27 | 426.15 | 443.32 |
20. | Ciprofloxacin | 2.04 | 20 | 332.18 | 288.22 | 245.15 |
21. | Cefacetrile | 2.09 | 300 | 362.07 | 258.08 | 178.01 |
22. | Tulathromycin A | 2.11 | 100 | 806.65 | 577.42 | 420.31 |
23. | Tetracycline | 2.21 | 25 | 445.28 | 154.07 | 427.32 |
24. | Danofloxacin | 2.23 | 20 | 358.22 | 340.16 | 314.21 |
25. | Enrofloxacin | 2.32 | 10 | 360.29 | 316.22 | 245.13 |
26. | Orbifloxacin | 2.35 | 10 | 396.22 | 352.17 | 226.12 |
27. | Thiamphenicol* | 2.38 | 200 | 354.16 | 290.04 | 184.98 |
28. | Sulfamethazine | 2.39 | 10 | 279.23 | 186.08 | 124.08 |
29. | Sulfamethizole | 2.52 | 10 | 271.17 | 156.02 | 108.02 |
30. | Sulfamethoxypyridazine | 2.56 | 10 | 281.14 | 156.03 | 126.07 |
31. | Sarafloxacin | 2.59 | 10 | 386.20 | 342.20 | 368.15 |
32. | Difloxacin | 2.65 | 10 | 400.23 | 356.17 | 299.13 |
33. | Cefazolin | 2.75 | 100 | 455.10 | 323.06 | 295.09 |
34. | Spiramycin | 2.96 | 200 | 843.64 | 540.36 | 699.48 |
35. | Pirlimycin | 3.05 | 20 | 411.32 | 363.18 | 327.21 |
36. | Chlortetracycline | 3.08 | 25 | 479.27 | 154.07 | 371.06 |
37. | Sulfachlorpyridazine | 3.12 | 20 | 285.05 | 156.03 | 108.09 |
38. | Gamithromycin | 3.28 | 100 | 777.63 | 619.52 | 601.45 |
39. | Sulfadoxine | 3.39 | 10 | 311.17 | 156.03 | 108.09 |
40. | Sulfamethoxazole | 3.46 | 20 | 254.18 | 155.98 | 147.06 |
41. | Cefoperazone | 3.52 | 100 | 646.26 | 143.07 | 148.02 |
42. | Florfenicol* | 3.55 | 200 | 356.10 | 336.02 | 184.98 |
43. | Sulfaethoxypyridazine | 3.56 | 20 | 295.17 | 267.07 | 156.03 |
44. | Tilmicosin | 3.64 | 100 | 869.72 | 696.50 | 522.42 |
45. | Sulfisoxazole | 3.76 | 20 | 268.14 | 156.03 | 113.10 |
46. | Oxolinic acid | 3.94 | 5 | 262.10 | 244.06 | 215.96 |
47. | Chloramphenicol* | 4.00 | 200 | 321.16 | 151.99 | 257.04 |
48. | Ceftiofur | 4.11 | 50 | 524.14 | 241.08 | 125.24 |
49. | Erythromycin | 4.31 | 25 | 734.64 | 576.40 | 558.38 |
50. | Sulfadimethoxine | 4.36 | 10 | 311.17 | 156.09 | 108.09 |
51. | Sulfaquinoxaline | 4.42 | 20 | 301.18 | 156.04 | 108.02 |
52. | Tylosin** | 4.67 | 100 | 916.62 | 772.49 | 598.36 |
53. | Penicillin G | 5.07 | 100 | 335.18 | 176.07 | 160.07 |
54. | Flumequine | 5.34 | 5 | 262.15 | 244.11 | 202.03 |
55. | Penicillin V | 5.56 | 100 | 351.10 | 160.06 | 114.07 |
56. | Oxacillin | 5.99 | 100 | 402.15 | 160.05 | 114.06 |
57. | Virginiamycin M1 | 6.13 | 50 | 526.43 | 508.31 | 355.10 |
58. | Tylvalosin | 6.19 | 50 | 1042.71 | 814.46 | 640.39 |
59. | Cloxacillin | 6.39 | 100 | 436.15 | 277.06 | 160.05 |
60. | Nafcillin | 6.60 | 25 | 415.19 | 199.09 | 171.06 |
61. | Dicloxacillin | 6.96 | 100 | 470.11 | 160.05 | 311.02 |
Column | Raptor C18 (cat.# 9304A12) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||||||
Guard Column: | Raptor C18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9304A0252) | ||||||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | Water | ||||||||||||||||||||||||
Conc.: | 5–300 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||||||
B: | 0.1% Formic acid in acetonitrile | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+/ESI- |
Mode: | Scheduled MRM |
Instrument | UHPLC |
Notes | 1. Positive and negative polarity data were collected simultaneously from a single injection. 2. Amphenicol compounds (chloramphenicol, thiamphenicol, and florfenicol) were detected with negative polarity. 3. The MRM was scheduled at +/- 20 to 30 seconds for each analyte. 4. Multiclass antibiotics include penicillin, cephalosporin, tetracycline, sulfonamide, macrolide, lincosamide, streptogramin, amphenicol, and quinolone. **The retention time for Tylosin is noted in the peak list; however, it was not included in the chromatogram. |