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Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | Polarity | |
---|---|---|---|---|---|---|
1. | Cyanuric acid | 0.47 | 127.8 | 84.9 | 42.1 | - |
2. | Cyromazine | 0.52 | 167.0 | 68.2 | 85.1 | + |
3. | Melamine | 0.89 | 127.2 | 85.0 | 68.3 | + |
4. | Ammelide | 2.18 | 129.1 | 86.1 | 70.2 | + |
5. | Ammeline | 2.97 | 128.2 | 86.2 | 69.1 | + |
Column | Raptor HILIC-Si (cat.# 9310A52) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||||||
Diluent: | 5:95 Water:acetonitrile, 10 mM ammonium formate, 0.1% formic acid | ||||||||||||||||||||||||
Conc.: | 25 ng/mL | ||||||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water, 10 mM ammonium formate, 0.1% formic acid | ||||||||||||||||||||||||
B: | 5:95 Water:acetonitrile, 10 mM ammonium formate, 0.1% formic acid | ||||||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+/ESI- |
Mode: | Scheduled MRM |
Instrument | HPLC |
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