LC Multiresidue Pesticide Mix Extracted from Brown Rice Flour with QuEChERS Slim Pouch on Raptor ARC-18 by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Cyromazine	0.747	167.1	68.1	43.1
2.	Methamidophos	1.318	142.0	94.0	125.1
3.	Formetanate	1.681	222.1	165.0	46.1
4.	Carbofuran	1.682	222.2	165.0	123.1
5.	Methabenzthiazuron	1.686	222.0	165.0	150.0
6.	Acephate	1.690	184.0	143.0	49.0
7.	Pymetrozine	1.708	218.1	105.0	78.0
8.	Aminocarb	1.716	209.1	137.1	152.1
9.	Propamocarb	1.735	189.1	102.0	144.1
10.	Omethoate	1.905	214.0	125.0	183.0
11.	Dinotefuran	1.972	203.1	129.1	87.1
12.	Aldicarb sulfoxide	2.010	207.1	89.0	132.1
13.	Butoxycarboxim	2.022	223.0	106.0	166.0
14.	Nitenpyram	2.049	271.0	225.0	56.0
15.	Aldicarb sulfone	2.062	240.2	148.0	86.0
16.	Carbendazim	2.090	192.0	160.0	131.9
17.	Oxamyl	2.154	237.1	72.0	90.0
18.	Flonicamid	2.234	230.1	203.0	148.0
19.	Methomyl	2.259	163.1	88.0	106.1
20.	Thiabendazole	2.284	201.8	92.2	104.0
21.	Thiamethoxam	2.289	292.1	211.1	181.1
22.	Fuberidazole	2.368	185.0	157.1	103.0
23.	Monocrotophos	2.375	224.1	127.0	193.1
24.	Mexacarbate	2.393	223.2	166.1	151.1
25.	Dioxacarb	2.397	224.1	167.0	77.1
26.	Dicrotophos	2.526	238.1	112.1	72.0
27.	Imidacloprid	2.536	256.1	175.0	209.0
28.	Clothianidin	2.545	250.0	169.0	113.0
29.	Dimethoate-d6 (IS)	2.652	236.2	205.2	131.2
30.	Fenuron	2.662	165.0	72.1	46.1
31.	Trichlorfon	2.667	257.0	108.9	220.8
32.	Dimethoate	2.668	230.0	199.0	125.0
33.	3-Hydroxycarbofuran	2.679	238.1	181.0	163.0
34.	Vamidothion	2.695	288.0	146.0	118.0
35.	Mevinphos	2.715	225.1	127.0	127.0
36.	Acetamiprid	2.724	223.0	126.0	56.1
37.	Propoxur	2.777	210.0	111.0	168.0
38.	Ethirimol	2.780	210.2	140.2	98.2
39.	Cymoxanil	2.805	199.1	128.2	111.2
40.	Thiacloprid	2.865	253.0	126.0	99.0
41.	Pirimicarb	2.896	239.2	72.0	182.1
42.	Aldicarb	3.022	208.2	116.2	89.2
43.	Tricyclazole	3.066	190.0	163.0	136.0
44.	Oxadixyl	3.076	279.1	219.1	132.1
45.	Carbetamide	3.085	237.1	192.0	118.1
46.	Simetryn	3.122	214.1	68.0	96.0
47.	Thiophanate-methyl	3.146	343.0	151.0	311.1
48.	Thidiazuron	3.150	221.1	102.1	127.9
49.	Bendiocarb	3.212	224.1	167.1	109.0
50.	Imazalil	3.225	297.1	159.0	41.1
51.	Prometon	3.227	226.1	142.1	184.0
52.	Metribuzin	3.230	215.1	187.1	131.0
53.	Secbumeton	3.232	226.2	170.2	100.2
54.	Terbumeton	3.235	226.1	114.1	170.1
55.	Sulfentrazone*	3.263	387.0	309.0	201.0
56.	Pyracarbolid	3.279	218.1	125.1	97.0
57.	Tebuthiuron	3.310	229.1	172.1	116.0
58.	Carbaryl	3.316	202.1	145.0	127.0
59.	Carboxin	3.326	236.1	143.0	87.1
60.	Monolinuron	3.373	215.1	126.0	148.0
61.	Fluometuron	3.381	233.1	72.1	46.1
62.	Diuron	3.382	233.1	72.1	46.0
63.	Ethiofencarb	3.406	226.0	107.0	164.0
64.	Ametryn	3.447	228.1	68.1	186.1
65.	Chlorotoluron	3.450	213.0	72.1	46.1
66.	Metobromuron	3.483	259.0	170.0	148.1
67.	Propham	3.500	180.1	120.2	77.1
68.	Flutriafol	3.508	302.0	70.1	123.0
69.	Methoprotryne	3.532	272.1	170.0	198.1
70.	Forchlorfenuron	3.536	248.1	129.0	93.0
71.	Isoprocarb	3.537	194.1	95.1	137.1
72.	Fenpropimorph	3.561	304.2	147.1	117.0
73.	Atrazine-d5 (SS)	3.575	221.2	179.2	101.3
74.	Isocarbophos	3.606	291.1	231.1	121.1
75.	Isoproturon	3.612	207.1	72.1	46.1
76.	Desmedipham	3.620	318.0	154.1	301.0
77.	Phenmedipham	3.623	318.1	108.1	93.1
78.	Spiroxamine	3.626	298.2	144.2	58.2
79.	Pyrimethanil	3.659	200.0	107.0	82.0
80.	Metalaxyl	3.691	280.1	220.1	192.1
81.	Chlorantraniliprole	3.730	484.0	452.8	285.8
82.	Cycluron	3.785	199.1	89.1	46.0
83.	Prometryn	3.830	242.1	158.0	200.2
84.	Linuron-d6 (SS)	3.843	255.1	160.1	185.2
85.	Linuron	3.854	249.0	160.0	182.0
86.	Thiofanox	3.876	242.1	57.2	184.0
87.	Fenobucarb	3.894	208.0	95.1	152.1
88.	Azoxystrobin	3.915	404.2	329.0	344.0
89.	Diethofencarb	3.924	268.1	226.1	124.0
90.	Ethofumesate	3.925	304.1	121.1	241.0
91.	Ethiprole	3.932	397.0	350.9	255.0
92.	Fludioxonil*	3.935	247.1	180.1	126.1
93.	Methiocarb	3.958	226.1	169.1	121.1
94.	Siduron	3.967	233.3	94.0	137.1
95.	Halofenozide	3.974	331.1	104.9	275.0
96.	Fenamidone	3.993	312.0	92.1	236.1
97.	Furalaxyl	3.995	302.1	242.1	95.0
98.	Acibenzolar-S-methyl	4.023	210.9	136.1	91.1
99.	Nuarimol	4.030	315.1	252.0	207.0
100.	Dimethomorph	4.044	388.2	300.9	165.1
101.	Boscalid	4.050	343.0	306.8	140.0
102.	Mandipropamid	4.067	412.1	328.1	125.0
103.	Flutolanil	4.079	324.1	262.0	242.0

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
104.	Promecarb	4.104	208.1	109.0	151.0
105.	Paclobutrazol	4.119	294.0	70.1	125.0
106.	Triadimenol	4.119	296.1	70.1	43.1
107.	Mepronil	4.161	270.1	91.1	119.0
108.	Cyproconazole	4.175	292.2	70.1	125.0
109.	Myclobutanil	4.195	289.2	70.1	125.1
110.	Chloroxuron	4.225	291.1	72.1	46.1
111.	Bupirimate	4.226	317.2	166.0	237.1
112.	Methoxyfenozide	4.230	369.2	149.0	313.1
113.	Triadimefon	4.287	294.0	69.0	197.0
114.	Bifenazate	4.290	301.1	198.0	170.1
115.	Cyprodinil	4.313	226.1	93.0	77.0
116.	Mefenacet	4.325	299.1	148.1	120.1
117.	Mepanipyrim	4.334	224.1	77.1	106.1
118.	Fluquinconazole	4.346	376.0	348.9	307.0
119.	Fenhexamid	4.383	302.1	97.1	55.1
120.	Fluoxastrobin	4.385	459.2	427.0	188.1
121.	Tetraconazole	4.387	372.0	159.0	70.0
122.	Bromuconazole isomer 1	4.392	376.0	159.0	159.0
123.	Butafenacil	4.411	492.1	330.9	179.9
124.	Iprovalicarb	4.412	321.2	119.0	91.1
125.	Flufenacet	4.427	364.1	152.0	194.1
126.	Triticonazole	4.429	318.0	70.0	125.0
127.	Cyazofamid	4.441	325.1	108.0	44.0
128.	Diflubenzuron	4.464	311.0	158.0	141.0
129.	Spirotetramat	4.478	374.1	330.1	302.0
130.	Fenarimol	4.510	331.0	267.9	189.1
131.	Fenbuconazole	4.516	337.0	125.0	70.1
132.	Epoxiconazole	4.529	330.0	121.1	101.0
133.	Etaconazole	4.552	328.1	159.0	55.0
134.	Fipronil*	4.571	436.9	332.0	252.0
135.	Neburon	4.635	275.0	57.1	88.1
136.	Flusilazole	4.646	316.1	247.1	165.1
137.	Fenoxycarb	4.653	302.2	88.1	116.1
138.	Picoxystrobin	4.674	368.0	145.0	205.0
139.	Rotenone	4.683	395.1	213.1	192.1
140.	Tebufenozide	4.719	353.2	133.1	105.0
141.	Dimoxystrobin	4.738	327.1	205.1	116.1
142.	Bromuconazole isomer 2	4.740	378.0	159.0	159.0
143.	Flubendiamide	4.745	700.0	408.0	274.0
144.	Diclobutrazol	4.764	328.0	70.1	159.0
145.	Carfentrazone ethyl	4.773	429.1	412.1	346.1
146.	Kresoxim-methyl	4.795	314.0	267.1	116.0
147.	Tebuconazole	4.827	308.0	70.1	124.9
148.	Spinosad A	4.840	732.5	142.1	98.1
149.	Penconazole	4.853	284.1	70.1	159.0
150.	Prothioconazole	4.857	344.0	189.0	154.0
151.	Zoxamide	4.893	336.1	187.0	159.0
152.	Famoxadone	4.933	392.2	331.1	238.0
153.	Diazinon-diethyl-d10 (IS)	4.961	315.2	170.2	154.3
154.	Hydramethylnon	4.979	495.2	323.2	45.2
155.	Triflumuron	4.986	359.1	156.0	111.0
156.	Prochloraz	4.986	376.0	308.0	70.1
157.	Hexaconazole	4.993	314.1	70.0	158.8
158.	Benalaxyl	5.021	326.2	148.0	208.1
159.	Metconazole	5.033	320.1	70.1	125.0
160.	Propiconazole	5.054	342.0	159.0	69.0
161.	Pyraclostrobin	5.076	388.0	194.0	163.1
162.	Clofentezine	5.080	303.0	138.0	102.0
163.	Bitertanol	5.120	338.2	269.2	70.1
164.	Spinosad D	5.142	746.5	142.0	98.0
165.	Benzoximate	5.171	364.0	199.1	105.0
166.	Diniconazole	5.184	326.1	70.0	158.9
167.	Thiobencarb	5.197	258.0	125.0	89.1
168.	Pencycuron	5.229	329.1	125.0	89.0
169.	Spinetoram	5.234	748.5	142.1	98.2
170.	Hexaflumuron*	5.351	459.0	439.0	175.0
171.	Triflumizole	5.366	346.1	278.0	73.1
172.	Indoxacarb	5.375	528.1	149.9	203.0
173.	Difenoconazole	5.380	406.1	251.1	152.0
174.	Ipconazole	5.426	334.2	70.0	124.9
175.	Trifloxystrobin	5.426	409.0	186.0	206.0
176.	Novaluron*	5.455	491.0	471.0	304.9
177.	Emamectin-benzoate B1b	5.463	872.2	158.2	82.1
178.	Clethodim	5.565	360.1	164.1	268.1
179.	Teflubenzuron	5.614	380.9	140.9	158.0
180.	Alanycarb	5.636	400.1	91.0	238.0
181.	Buprofezin	5.642	306.2	106.1	86.0
182.	Emamectin-benzoate B1a	5.644	886.5	158.0	82.1
183.	Benfuracarb	5.695	411.2	194.9	251.9
184.	Fluazinam*	5.726	463.0	415.9	397.9
185.	Metaflumizone*	5.728	505.1	302.1	328.0
186.	Furathiocarb	5.735	383.1	195.0	252.1
187.	Temephos	5.757	467.1	125.0	418.9
188.	Lufenuron	5.767	511.2	141.0	158.0
189.	Tebufenpyrad	5.778	334.0	117.0	145.0
190.	Pyriproxyfen	5.843	322.0	96.0	227.1
191.	Piperonyl butoxide	5.864	356.2	177.0	119.1
192.	Hexythiazox	5.924	353.2	228.0	168.1
193.	Quinoxyfen	5.935	308.1	197.0	162.0
194.	Flufenoxuron	5.984	489.1	158.0	141.0
195.	Propargite	6.088	368.1	231.1	175.0
196.	Etoxazole	6.099	360.2	141.0	57.1
197.	Spiromesifen	6.129	371.3	273.2	255.2
198.	Chlorfluazuron	6.165	539.8	382.9	158.0
199.	Spirodiclofen	6.269	428.3	71.1	313.1
200.	(E)-Fenpyroximate	6.283	422.1	366.1	138.1
201.	Pyridaben	6.442	365.2	147.0	309.1
202.	Eprinomectin	6.466	914.6	186.1	154.1
203.	Abamectin B1a	6.544	890.6	305.4	567.2
204.	Fenazaquin	6.559	307.1	161.0	131.1
205.	Doramectin	6.733	916.6	331.2	593.4
206.	Ivermectin	6.893	892.6	551.4	569.4

*Detected in ESI- mode

Column

Raptor ARC-18 (cat.# 9314A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

50 °C

Standard/Sample

LC multiresidue pesticide kit (cat.# 31971)

Diluent:

Water:acetonitrile 9:1

Conc.:

1 ng/mL see notes for details

Inj. Vol.:

5 µL

Mobile Phase

0.2% Formic acid, 2 mM ammonium formate in water

0.2% Formic acid, 2 mM ammonium formate in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	95	5
2.00	0.4	40	60
4.00	0.4	25	75
6.00	0.4	0	100
7.50	0.4	0	100
7.51	0.4	95	5
9.50	0.4	95	5

Detector	MS/MS
Ion Mode:	ESI+/ESI-
Mode:	Scheduled MRM
Instrument	UHPLC
Sample Preparation	Brown rice flour (10 g), purchased from a local market, was mixed with 10 mL water. After mixing for 10 min, the brown rice flour was fortified at 10 ng/g with all residues from the LC multiresidue pesticide kit (cat.# 31971), which had been previously combined into a single mixture that day. The fortified brown rice flour suspension was then mixed for 30 min to ensure uniform dispersion of the added pesticide residues in the brown rice flour suspension. Extraction was performed using 10 mL of acetonitrile and original unbuffered QuEChERS salts (cat.# 25848). After centrifugation, 1 mL supernatant was added to a 2 mL dSPE vial containing magnesium sulfate, PSA, and C18 (cat.# 26125) for cleanup. After centrifugation, 100 μL of the 10 ng/mL extract was diluted with 900 μL water and injected onto the UHPLC.
Notes	Although the ten LC multiresidue pesticides kit mixes are formulated to ensure maximum long-term stability and reliability as packaged, stability may become an issue when a large number of compounds with different chemical functionalities are combined together into a single mix. This should be taken into consideration for quantitative analysis.

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