LC Multiresidue Pesticide Mix Extracted from Celery with QuEChERS Slim Pouch on Raptor ARC-18 by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Cyromazine	0.749	167.1	68.1	43.1
2.	Methamidophos	1.314	142.0	94.0	125.1
3.	Formetanate	1.684	222.1	165.0	46.1
4.	Carbofuran	1.685	222.2	165.0	123.1
5.	Methabenzthiazuron	1.685	222.0	165.0	150.0
6.	Acephate	1.692	184.0	143.0	49.0
7.	Pymetrozine	1.705	218.1	105.0	78.0
8.	Aminocarb	1.711	209.1	137.1	152.1
9.	Propamocarb	1.731	189.1	102.0	144.1
10.	Omethoate	1.899	214.0	125.0	183.0
11.	Dinotefuran	1.964	203.1	129.1	87.1
12.	Aldicarb sulfoxide	2.005	207.1	89.0	132.1
13.	Butoxycarboxim	2.016	223.0	106.0	166.0
14.	Nitenpyram	2.044	271.0	56.0	56.0
15.	Aldicarb sulfone	2.055	240.2	148.0	86.0
16.	Carbendazim	2.084	192.0	160.0	131.9
17.	Oxamyl	2.150	237.1	72.0	90.0
18.	Flonicamid	2.230	230.1	203.0	148.0
19.	Methomyl	2.258	163.1	88.0	106.1
20.	Thiabendazole	2.273	201.8	104.0	104.0
21.	Thiamethoxam	2.283	292.1	211.1	181.1
22.	Fuberidazole	2.362	185.0	157.1	103.0
23.	Monocrotophos	2.373	224.1	127.0	193.1
24.	Dioxacarb	2.374	224.1	167.0	77.1
25.	Mexacarbate	2.377	223.2	151.1	151.1
26.	Dicrotophos	2.520	238.1	112.1	72.0
27.	Imidacloprid	2.532	256.1	175.0	209.0
28.	Clothianidin	2.541	250.0	169.0	113.0
29.	Dimethoate-d6 (IS)	2.650	236.2	205.2	131.2
30.	Fenuron	2.660	165.0	72.1	46.1
31.	Trichlorfon	2.664	257.0	108.9	220.8
32.	Dimethoate	2.666	230.0	125.0	125.0
33.	3-Hydroxycarbofuran	2.680	238.1	163.0	163.0
34.	Vamidothion	2.693	288.0	146.0	118.0
35.	Mevinphos	2.710	225.1	127.0	127.0
36.	Acetamiprid	2.720	223.0	126.0	56.1
37.	Propoxur	2.765	210.0	111.0	168.0
38.	Ethirimol	2.768	210.2	140.2	98.2
39.	Cymoxanil	2.802	199.1	128.2	111.2
40.	Thiacloprid	2.862	253.0	126.0	99.0
41.	Pirimicarb	2.871	239.2	72.0	182.1
42.	Butocarboxim	2.993	191.2	75.3	41.3
43.	Aldicarb	3.020	208.2	89.2	89.2
44.	Tricyclazole	3.062	190.0	136.0	136.0
45.	Oxadixyl	3.073	279.1	219.1	132.1
46.	Carbetamide	3.078	237.1	118.1	118.1
47.	Simetryn	3.103	214.1	68.0	96.0
48.	Thiophanate-methyl	3.144	343.0	151.0	311.1
49.	Thidiazuron	3.147	221.1	102.1	127.9
50.	Bendiocarb	3.203	224.1	109.0	109.0
51.	Prometon	3.206	226.1	142.1	184.0
52.	Secbumeton	3.210	226.2	100.2	100.2
53.	Imazalil	3.216	297.1	159.0	41.1
54.	Terbumeton	3.217	226.1	114.1	170.1
55.	Metribuzin	3.226	215.1	187.1	131.0
56.	Pyracarbolid	3.274	218.1	125.1	97.0
57.	Tebuthiuron	3.307	229.1	172.1	116.0
58.	Carbaryl	3.309	202.1	145.0	127.0
59.	Carboxin	3.321	236.1	143.0	87.1
60.	Monolinuron	3.371	215.1	126.0	148.0
61.	Fluometuron	3.378	233.1	72.1	46.1
62.	Diuron	3.380	233.1	72.1	46.0
63.	Ethiofencarb	3.400	226.0	107.0	164.0
64.	Ametryn	3.419	228.1	68.1	186.1
65.	Chlorotoluron	3.446	213.0	72.1	46.1
66.	Metobromuron	3.479	259.0	170.0	148.1
67.	Propham	3.496	180.1	77.1	77.1
68.	Flutriafol	3.504	302.0	70.1	123.0
69.	Methoprotryne	3.508	272.1	170.0	198.1
70.	Forchlorfenuron	3.535	248.1	93.0	93.0
71.	Isoprocarb	3.540	194.1	95.1	137.1
72.	Fenpropimorph	3.549	304.2	147.1	117.0
73.	Atrazine-d5 (SS)	3.571	221.2	179.2	101.3
74.	Isocarbophos	3.602	291.1	231.1	121.1
75.	Isoproturon	3.609	207.1	72.1	46.1
76.	Desmedipham	3.616	318.0	154.1	301.0
77.	Phenmedipham	3.617	318.1	108.1	93.1
78.	Spiroxamine	3.622	298.2	144.2	58.2
79.	Pyrimethanil	3.639	200.0	82.0	82.0
80.	Metalaxyl	3.688	280.1	220.1	192.1
81.	Chlorantraniliprole	3.731	484.0	452.8	285.8
82.	Cycluron	3.780	199.1	89.1	46.0
83.	Prometryn	3.798	242.1	158.0	200.2
84.	Linuron-d6 (SS)	3.839	255.1	160.1	185.2
85.	Linuron	3.851	249.0	160.0	182.0
86.	Fenobucarb	3.885	208.0	95.1	152.1
87.	Azoxystrobin	3.914	404.2	372.0	344.0
88.	Diethofencarb	3.924	268.1	226.1	124.0
89.	Fludioxonil*	3.927	247.1	180.1	126.1
90.	Ethofumesate	3.928	304.1	121.1	241.0
91.	Ethiprole	3.936	397.0	255.0	255.0
92.	Methiocarb	3.956	226.1	121.1	121.1
93.	Siduron	3.962	233.3	94.0	137.1
94.	Halofenozide	3.972	331.1	104.9	275.0
95.	Fenamidone	3.986	312.0	92.1	236.1
96.	Furalaxyl	3.989	302.1	95.0	95.0
97.	Acibenzolar-S-methyl	4.018	210.9	136.1	91.1
98.	Nuarimol	4.027	315.1	252.0	207.0
99.	Dimethomorph	4.040	388.2	300.9	165.1
100.	Boscalid	4.048	343.0	306.8	140.0
101.	Mandipropamid	4.065	412.1	328.1	125.0

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
102.	Flutolanil	4.074	324.1	242.0	242.0
103.	Promecarb	4.092	208.1	109.0	151.0
104.	Triadimenol	4.114	296.1	70.1	43.1
105.	Paclobutrazol	4.115	294.0	70.1	125.0
106.	Mepronil	4.158	270.1	119.0	119.0
107.	Cyproconazole	4.169	292.2	70.1	125.0
108.	Bupirimate	4.186	317.2	166.0	237.1
109.	Myclobutanil	4.192	289.2	70.1	125.1
110.	Chloroxuron	4.220	291.1	72.1	46.1
111.	Methoxyfenozide	4.225	369.2	149.0	313.1
112.	Cyprodinil	4.261	226.1	93.0	77.0
113.	Triadimefon	4.281	294.0	69.0	197.0
114.	Bifenazate	4.291	301.1	170.1	170.1
115.	Mefenacet	4.323	299.1	120.1	120.1
116.	Mepanipyrim	4.329	224.1	106.1	106.1
117.	Fluquinconazole	4.342	376.0	348.9	307.0
118.	Fluoxastrobin	4.376	459.2	427.0	188.1
119.	Tetraconazole	4.379	372.0	159.0	70.0
120.	Fenhexamid	4.382	302.1	97.1	55.1
121.	Bromuconazole isomer 1	4.382	376.0	159.0	159.0
122.	Iprovalicarb	4.408	321.2	119.0	91.1
123.	Butafenacil	4.408	492.1	179.9	179.9
124.	Flufenacet	4.424	364.1	152.0	194.1
125.	Triticonazole	4.427	318.0	70.0	125.0
126.	Cyazofamid	4.431	325.1	108.0	44.0
127.	Diflubenzuron	4.451	311.0	158.0	141.0
128.	Spirotetramat	4.478	374.1	330.1	302.0
129.	Fenarimol	4.509	331.0	267.9	189.1
130.	Fenbuconazole	4.514	337.0	125.0	70.1
131.	Epoxiconazole	4.525	330.0	121.1	101.0
132.	Etaconazole	4.553	328.1	159.0	55.0
133.	Fipronil*	4.573	436.9	332.0	252.0
134.	Neburon	4.634	275.0	88.1	88.1
135.	Flusilazole	4.640	316.1	165.1	165.1
136.	Fenoxycarb	4.647	302.2	88.1	116.1
137.	Picoxystrobin	4.671	368.0	145.0	205.0
138.	Rotenone	4.679	395.1	213.1	192.1
139.	Tebufenozide	4.715	353.2	133.1	105.0
140.	Dimoxystrobin	4.732	327.1	116.1	116.1
141.	Bromuconazole isomer 2	4.740	378.0	159.0	159.0
142.	Flubendiamide	4.744	700.0	408.0	274.0
143.	Diclobutrazol	4.759	328.0	70.1	159.0
144.	Carfentrazone ethyl	4.768	429.1	412.1	346.1
145.	Kresoxim-methyl	4.796	314.0	116.0	116.0
146.	Tebuconazole	4.821	308.0	70.1	124.9
147.	Spinosad A	4.833	732.5	142.1	98.1
148.	Penconazole	4.848	284.1	70.1	159.0
149.	Zoxamide	4.891	336.1	187.0	159.0
150.	Famoxadone	4.937	392.2	238.0	238.0
151.	Prochloraz	4.954	376.0	308.0	70.1
152.	Diazinon-diethyl-d10 (IS)	4.955	315.2	170.2	154.3
153.	Triflumuron	4.982	359.1	156.0	111.0
154.	Hydramethylnon	4.984	495.2	323.2	45.2
155.	Hexaconazole	4.991	314.1	70.0	158.8
156.	Benalaxyl	5.016	326.2	148.0	208.1
157.	Metconazole	5.030	320.1	70.1	125.0
158.	Propiconazole	5.053	342.0	159.0	69.0
159.	Pyraclostrobin	5.070	388.0	163.1	163.1
160.	Clofentezine	5.075	303.0	138.0	102.0
161.	Bitertanol	5.114	338.2	70.1	70.1
162.	Spinosad D	5.139	746.5	142.0	98.0
163.	Benzoximate	5.169	364.0	105.0	105.0
164.	Diniconazole	5.182	326.1	70.0	158.9
165.	Thiobencarb	5.192	258.0	125.0	89.1
166.	Spinetoram	5.220	748.5	142.1	98.2
167.	Pencycuron	5.225	329.1	125.0	89.0
168.	Triflumizole	5.340	346.1	278.0	73.1
169.	Hexaflumuron*	5.342	459.0	439.0	175.0
170.	Indoxacarb	5.370	528.1	203.0	203.0
171.	Difenoconazole	5.377	406.1	251.1	152.0
172.	Trifloxystrobin	5.419	409.0	186.0	206.0
173.	Ipconazole	5.421	334.2	70.0	124.9
174.	Emamectin-benzoate B1b	5.441	872.2	158.2	82.1
175.	Novaluron*	5.443	491.0	471.0	304.9
176.	Clethodim	5.559	360.1	164.1	268.1
177.	Teflubenzuron	5.610	380.9	140.9	158.0
178.	Buprofezin	5.618	306.2	106.1	86.0
179.	Emamectin-benzoate B1a	5.632	886.5	158.0	82.1
180.	Alanycarb	5.684	400.1	91.0	238.0
181.	Fluazinam*	5.719	463.0	415.9	397.9
182.	Metaflumizone*	5.720	505.1	302.1	328.0
183.	Furathiocarb	5.731	383.1	195.0	252.1
184.	Temephos	5.753	467.1	125.0	418.9
185.	Lufenuron	5.771	511.2	141.0	158.0
186.	Tebufenpyrad	5.775	334.0	117.0	145.0
187.	Pyriproxyfen	5.834	322.0	96.0	227.1
188.	Piperonyl butoxide	5.858	356.2	177.0	119.1
189.	Hexythiazox	5.922	353.2	168.1	168.1
190.	Quinoxyfen	5.924	308.1	197.0	162.0
191.	Flufenoxuron	5.980	489.1	141.0	141.0
192.	Propargite	6.084	368.1	231.1	175.0
193.	Etoxazole	6.090	360.2	141.0	57.1
194.	Spiromesifen	6.124	371.3	273.2	255.2
195.	Chlorfluazuron	6.157	539.8	382.9	158.0
196.	Spirodiclofen	6.263	428.3	71.1	313.1
197.	Fenpyroximate	6.277	422.1	366.1	138.1
198.	Pyridaben	6.433	365.2	147.0	309.1
199.	Eprinomectin	6.463	914.6	186.1	154.1
200.	Abamectin B1a	6.541	890.6	305.4	567.2
201.	Fenazaquin	6.549	307.1	161.0	131.1
202.	Doramectin	6.727	916.6	331.2	593.4
203.	Ivermectin	6.884	892.6	551.4	569.4

*Detected in ESI- mode

Column

Raptor ARC-18 (cat.# 9314A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

50 °C

Standard/Sample

LC multiresidue pesticide kit (cat.# 31971)

Diluent:

Water:acetonitrile 9:1

Conc.:

1 ng/mL see notes for more details

Inj. Vol.:

5 µL

Mobile Phase

0.2% Formic acid, 2 mM ammonium formate in water

0.2% Formic acid, 2 mM ammonium formate in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	95	5
2.00	0.4	40	60
4.00	0.4	25	75
6.00	0.4	0	100
7.50	0.4	0	100
7.51	0.4	95	5
9.50	0.4	95	5

Detector	MS/MS
Ion Mode:	ESI+/ESI-
Mode:	Scheduled MRM
Instrument	UHPLC
Notes	Although the ten LC multiresidue pesticides kit mixes are formulated to ensure maximum long-term stability and reliability as packaged, stability may become an issue when a large number of compounds with different chemical functionalities are combined together into a single mix. This should be taken into consideration for quantitative analysis. Sample Preparation: Celery purchased from a local market was comminuted using a Robot Coup Blixer 3. After comminution, 15 g celery was fortified at 10 ng/g with all residues from the LC multiresidue pesticide kit (cat.# 31971), which had been previously combined into a single mixture that day. The fortified celery was then shaken for a couple minutes to ensure uniform dispersion of the fortified pesticides in the slurry. Extraction was performed by adding 15 mL of a 1% acetic acid solution in acetonitrile (v/v) and AOAC 2007.01 QuEChERS salts (cat.# 25852). After centrifugation, 1 mL supernatant was added to a 2 mL dSPE vial containing magnesium sulfate, PSA, and GCB (cat.# 26218) for cleanup. After centrifugation, 100 μL of the 10 ng/mL extract was diluted with 900 μL water and injected onto the UHPLC.

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