Mycotoxins in Solvent on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/g)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Deoxynivalenol	0.64	150	297.3	249.3	231.2
2.	Deoxynivalenol-13C15	0.64	125	312.3	263.3	245.2
3.	Fumonisin B1	2.45	150	722.5	352.4	334.5
4.	HT-2	2.59	150	447.3	285.3	345.3
5.	Fumonisin B3	2.85	150	706.5	336.4	318.4
6.	Fumonisin B2	3.23	150	706.5	336.3	141.2
7.	T-2	3.31	150	489.3	245.2	387.4
8.	Aflatoxin G2	3.74	15	331.2	313.3	189.3
9.	Aflatoxin G2-13C17	3.74	25	348.3	330.3	200.4

	Peaks	t_R (min)	Conc. (ng/g)	Precursor Ion	Product Ion 1	Product Ion 2
10.	Zearalenone	3.96	150	319.3	283.3	187.2
11.	Aflatoxin G1	4.22	15	329.2	243.2	200.2
12.	Aflatoxin G1-13C17	4.22	25	346.3	257.3	212.3
13.	Aflatoxin B2	4.43	15	315.3	287.3	259.2
14.	Aflatoxin B2-13C17	4.43	25	332.3	303.3	273.3
15.	Aflatoxin B1	4.99	15	313.3	285.2	241.2
16.	Aflatoxin B1-13C17	4.99	25	330.3	301.4	255.3
17.	Ochratoxin A	5.19	15	404.2	239.3	358.3
18.	Ochratoxin A-13C20	5.19	20	424.3	250.2	377.2

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

40 °C

Standard/Sample

Diluent:

65:35 Water:methanol

Inj. Vol.:

5 µL

Mobile Phase

Water, 2 mM ammonium formate, 0.1% formic acid

Methanol, 2 mM ammonium formate, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	70	30
0.6	0.5	70	30
0.7	0.5	50	50
3.0	0.5	30	70
4.5	0.5	25	75
5.0	0.5	10	90
5.2	0.5	10	90
5.21	0.5	25	75
6.00	0.5	25	75
6.01	0.5	70	30
7.00	0.5	70	30

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