Mycotoxins in Yellow Corn Meal on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Conc. (ng/g)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Deoxynivalenol	0.62	50	297.3	249.3	231.2
2.	Fumonisin B1	2.45	50	722.5	352.4	334.5
3.	HT-2	2.60	50	447.3	285.3	345.3
4.	Fumonisin B3	2.85	50	706.5	336.4	318.4
5.	Fumonisin B2	3.23	50	706.5	336.3	141.2
6.	T2	3.31	50	489.3	245.2	387.4

	Peaks	t_R (min)	Conc. (ng/g)	Precursor Ion	Product Ion 1	Product Ion 2
7.	Aflatoxin G2	3.74	5	331.2	313.3	189.3
8.	Zearalenone	3.96	50	319.3	283.3	187.2
9.	Aflatoxin G1	4.22	5	329.2	243.2	200.2
10.	Aflatoxin B2	4.43	5	315.3	287.3	259.2
11.	Aflatoxin B1	4.99	5	313.3	285.2	241.2
12.	Ochratoxin A	5.19	5	404.2	239.3	358.3

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

40 °C

Inj. Vol.:

5 µL

Mobile Phase

Water, 2 mM ammonium formate, 0.1% formic acid

Methanol, 2 mM ammonium formate, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	70	30
0.6	0.5	70	30
0.7	0.5	50	50
3.0	0.5	30	70
4.5	0.5	25	75
5.0	0.5	10	90
5.2	0.5	10	90
5.21	0.5	25	75
6.00	0.5	25	75
6.01	0.5	70	30
7.00	0.5	70	30

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	Weighed 1.00 gram of corn meal in a 50-mL centrifuge tube and added 2.00 mL of water. Vortexed at 3000 rpm for 5 min followed by the addition of 4.0 mL of extraction solvent (50:50 water:acetonitrile, v/v). The tube was then vortexed at 3000 rpm for 5 min followed by centrifugation for 15 min at 4200 rpm. 475 μL of the supernatant was filtered through a Thomson SINGLE StEP Nano filter vial (0.2 μm, cat.# 25882). The sample was then fortified with 25 μL of a standard solution prepared in water at 1000 ng/mL (100 ng/mL for aflatoxins and orchratoxin A) as part of the matrix-matched calibration curve. Vortexed at 3000 rpm for 1 min prior to analysis.

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