Sulfur Antibiotics on Ultra Biphenyl

LC_GN0531

	Peaks	t_R (min)
1.	Sulfanilamide	4.40
2.	Sulfadiazine	10.18
3.	Sulfapyridine	10.63
4.	Sulfathiazole	10.99
5.	Sulfamerazine	11.72

	Peaks	t_R (min)
6.	Sulfamethazine	12.94
7.	Sulfachlorpyridazine	16.08
8.	Sulfadoxine	16.42
9.	Sulfisoxazole	17.65
10.	Sulfadimethoxine	19.47
11.	Sulfaquinoxaline	19.86

Column

Ultra Biphenyl (cat.# 9109565)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

5 µm

Pore Size:

100 Å

Temp.:

25 °C

Standard/Sample

Diluent:

0.1% Formic acid in water

Conc.:

50 µg/mL

Inj. Vol.:

10 µL

Mobile Phase

A:

0.1% Formic acid in water

B:

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	1	90	10
3.0	1	90	10
20.0	1	60	40
21.0	1	60	40

Detector	UV/Vis @ 265 nm
Preconcentrator
Instrument	Shimadzu UFLCXR

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