16 Cannabinoids on Raptor ARC-18 1.8 μm by LC-UV

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	0.787
2.	Cannabidivarin (CBDV)	0.867
3.	Cannabidiolic acid (CBDA)	1.090
4.	Cannabigerolic acid (CBGA)	1.157
5.	Cannabigerol (CBG)	1.216
6.	Cannabidiol (CBD)	1.279
7.	Tetrahydrocannabivarin (THCV)	1.396
8.	Tetrahydrocannabivarinic acid (THCVA)	1.774

	Peaks	t_R (min)
9.	Cannabinol (CBN)	1.894
10.	Cannabinolic acid (CBNA)	2.257
11.	Δ9-Tetrahydrocannabinol (Δ9-THC)	2.380
12.	Δ8-Tetrahydrocannabinol (Δ8-THC)	2.456
13.	Cannabicyclol (CBL)	2.826
14.	Cannabichromene (CBC)	2.966
15.	Tetrahydrocannabinolic acid A (THCA-A)	3.150
16.	Cannabichromenic acid (CBCA)	3.516

Column

Raptor ARC-18 (cat.# 931421E)

Dimensions:

100 mm x 3.0 mm ID

Particle Size:

1.8 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)

Temp.:

30 °C

Standard/Sample

Tetrahydrocannabivarin (cat.# 34100)

Cannabidiolic acid (CBDA) (cat.# 34099)

Cannabichromene (CBC) (cat.# 34092)

Cannabigerol (CBG) (cat.# 34091)

delta-9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34093)

delta-8-Tetrahydrocannabinol (Δ⁸-THC) (cat.# 34090)

delta-9-Tetrahydrocannabinol (Δ⁹-THC) (cat.# 34067)

Cannabinol (CBN) (cat.# 34010)

Cannabidiol (CBD) (cat.# 34011)

Compounds not present in these mixes were obtained separately.

Diluent:

25:75 Water:methanol

Conc.:

50 µg/mL

Inj. Vol.:

1 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.0	25	75
4.00	1.0	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC

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