Potency Analysis of a Commercially Available CBD Product on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)
1.	Cannabidiolic acid (CBDA)	2.608
2.	Cannabigerol (CBG)	2.934
3.	Cannabidiol (CBD)	3.101
4.	Δ9-Tetrahydrocannabinol (Δ9-THC)	5.851
5.	Cannabichromene (CBC)	7.315

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Diluent:

25:75 Water:methanol

Conc.:

6.24 mg/mL (CBD)

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
9.00	1.5	25	75

Detector	UV/Vis @ 228 nm
Instrument	HPLC
Sample Preparation	50 μL of a commerically available CBD product was diluted in 950 μL of 25:75 water:methanol. The sample was vortexed for 30 seconds at 3000 rpm. 400 μL of the sample was then filtered using a Thomson SINGLE StEP standard filter vial (PVDF, 0.2 μm, Restek cat.# 25895) prior to analysis.
Notes	CBDA, CBG, CBD, Δ9-THC, and CBC were detected, but only CBD was quantified.

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