Mycotoxins (STD 1 - 2 ng/g) in MCT Oil on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion 1	Product Ion 2
1.	Aflatoxin G2-13C17	1.340	348.3	330.3	-
2.	Aflatoxin G2	1.344	331.2	189.3	115.2
3.	Aflatoxin G1-13C17	1.576	346.3	257.3	-
4.	Aflatoxin G1	1.579	329.2	243.2	215.3
5.	Aflatoxin B2-13C17	1.707	332.3	303.3	-
6.	Aflatoxin B2	1.711	315.3	287.2	243.3
7.	Ochratoxin A	1.824	404.3	239.1	358.3
8.	Ochratoxin A-13C20	1.825	424.3	250.2	-
9.	Aflatoxin B1	1.946	313.2	241.2	128.2
10.	Aflatoxin B1-13C17	1.948	330.3	301.4	-

Column

Raptor Biphenyl (cat.# 9309A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)

Temp.:

35 °C

Sample

Diluent:

45:55 Water:Methanol

Conc.:

2 ng/g

Inj. Vol.:

5 µL

Mobile Phase

Water, 2 mM ammonium formate, 0.1% formic acid

Methanol, 2 mM ammonium formate, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.7	35	65
2.00	0.7	10	90
2.01	0.7	35	65
3.00	0.7	35	65

Detector	MS/MS
Ion Mode:	ESI+
Mode:	MRM
Instrument	UHPLC
Notes	A working calibration solution containing Aflatoxin B1, B2, G1, and G2 and Ochratoxin A was prepared at 50.0 ng/mL in methanol. 0.25 g of MCT oil was spiked to 2 ng/g using 10 µL of the working calibration solution. A working internal standard was prepared using 13C labeled analogs at a concentration of 250 ng/mL in methanol. 10 µL of the working internal standard was aliquoted into the sample followed by vortexing for 10 seconds at 3,000 rpm. 1 mL of 45:55 H₂O:MeOH was added to the sample. The sample was vortexed for 30 seconds at 3,000 rpm. The sample was then centrifuged at 3,000 xg for 5 min. at 10 °C. 750 µL of the supernatant was transferred to a conditioned (1 mL 45:55 water:methanol) Resprep bonded reversed phase SPE cartridge (Restek cat.# 26030). The sample was pulled through under vacuum into an autosampler vial for LC-MS/MS analysis.

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