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|Peaks||tR (min)||Precursor Ion||Product Ion 1||Product Ion 2|
|Column||Raptor Biphenyl (cat.# 9309A52)|
|Dimensions:||50 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)|
|Inj. Vol.:||5 µL|
|A:||Water, 2 mM ammonium formate, 0.1% formic acid|
|B:||Methanol, 2 mM ammonium formate, 0.1% formic acid|
|Sample Preparation||A working calibration solution containing Aflatoxin B1, B2, G1, and G2 and Ochratoxin A was prepared at 50.0 ng/mL in methanol. 0.25 g of MCT oil was spiked to 2 ng/g using 10 µL of the working calibration solution. A working internal standard was prepared using 13C labeled analogs at a concentration of 250 ng/mL in methanol. 10 µL of the working internal standard was aliquoted into the sample followed by vortexing for 10 seconds at 3000 rpm. 1 mL of 45:55 H2O:MeOH was added to the sample. The sample was vortexed for 30 seconds at 3000 rpm. The sample was then centrifuged at 3000 xg for 5 min. at 10 °C. 750 µL of the supernatant was transferred to a conditioned (1 mL 45:55 water:methanol) Resprep bonded reversed phase SPE cartridge (Restek cat.# 26030). The sample was pulled through under vacuum into an autosampler vial for LC-MS/MS analysis.|