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Peaks | tR (min) | Precursor Ion | Product Ion 1 | Product Ion 2 | |
---|---|---|---|---|---|
1. | Aflatoxin G2-13C17 | 1.340 | 348.3 | 330.3 | - |
2. | Aflatoxin G2 | 1.344 | 331.2 | 189.3 | 115.2 |
3. | Aflatoxin G1-13C17 | 1.576 | 346.3 | 257.3 | - |
4. | Aflatoxin G1 | 1.579 | 329.2 | 243.2 | 215.3 |
5. | Aflatoxin B2-13C17 | 1.707 | 332.3 | 303.3 | - |
6. | Aflatoxin B2 | 1.711 | 315.3 | 287.2 | 243.3 |
7. | Ochratoxin A | 1.824 | 404.3 | 239.1 | 358.3 |
8. | Ochratoxin A-13C20 | 1.825 | 424.3 | 250.2 | - |
9. | Aflatoxin B1 | 1.946 | 313.2 | 241.2 | 128.2 |
10. | Aflatoxin B1-13C17 | 1.948 | 330.3 | 301.4 | - |
Column | Raptor Biphenyl (cat.# 9309A52) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||
Standard/Sample | |||||||||||||||||||||
Diluent: | 45:55 Water:Methanol | ||||||||||||||||||||
Conc.: | 2 ng/g | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | Water, 2 mM ammonium formate, 0.1% formic acid | ||||||||||||||||||||
B: | Methanol, 2 mM ammonium formate, 0.1% formic acid | ||||||||||||||||||||
|
Detector | MS/MS |
---|---|
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | UHPLC |
Sample Preparation | A working calibration solution containing Aflatoxin B1, B2, G1, and G2 and Ochratoxin A was prepared at 50.0 ng/mL in methanol. 0.25 g of MCT oil was spiked to 2 ng/g using 10 µL of the working calibration solution. A working internal standard was prepared using 13C labeled analogs at a concentration of 250 ng/mL in methanol. 10 µL of the working internal standard was aliquoted into the sample followed by vortexing for 10 seconds at 3000 rpm. 1 mL of 45:55 H2O:MeOH was added to the sample. The sample was vortexed for 30 seconds at 3000 rpm. The sample was then centrifuged at 3000 xg for 5 min. at 10 °C. 750 µL of the supernatant was transferred to a conditioned (1 mL 45:55 water:methanol) Resprep bonded reversed phase SPE cartridge (Restek cat.# 26030). The sample was pulled through under vacuum into an autosampler vial for LC-MS/MS analysis. |
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